Structural Adaptation of Cold-Active RTX Lipase from Pseudomonas sp. Strain AMS8 Revealed via Homology and Molecular Dynamics Simulation Approaches

Table 1

Ramachandran plot scores for LipAMS8 after simulation for 12 ns at different temperatures.

Temperature	0°C	5°C	25°C	37°C	50°C	100°C
Most favored regions (%)	85.1	83.1	87.2	81.3	84.4	82.1
Additional allowed regions (%)	13.1	15.9	12.3	17.7	14.9	16.2
Generously allowed regions (%)	1.5	0.8	0.3	0.3	0.0	1.3
Disallowed regions (%)	0.3	0.3	0.3	0.8	0.8	0.5
Nonglycine and nonproline residues (%)	100.0	100.0	100.0	100.0	100.0	100.0
Endresidues (excl. Gly and Pro)	2	2	2	2	2	2
Glycine residues	69	69	69	69	69	69
Proline residues	15	15	15	15	15	15
Total number of residues	476	476	476	476	476	476