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BioMed Research International
Volume 2013 (2013), Article ID 926942, 13 pages
2-Heptyl-Formononetin Increases Cholesterol and Induces Hepatic Steatosis in Mice
1Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark
2Department of Chemistry, Faculty of Science, University of Copenhagen, 1870 Frederiksberg C, Denmark
3Department of Food Science, Faculty of Science, University of Copenhagen, 1870 Frederiksberg C, Denmark
4Department of Biology, Faculty of Science, University of Copenhagen, 2200 Copenhagen N, Denmark
5Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen Ø, Denmark
Received 2 January 2013; Revised 15 March 2013; Accepted 26 March 2013
Academic Editor: Kazim Husain
Copyright © 2013 Charlotte Andersen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Supplementary Table 1: Composition of the diets.
Supplementary Table 2: A list of functional gene groupings included in the gene expression array analysis of phase I and II enzymes on C57BL/6 mice fed cholesterol supplemented formononetin or 2-heptyl formononetin (CF7) for three weeks (experiment 1).
Supplementary Table 3: Primer sequences.
Supplementary Figure 1: Clustering of gut microbiota from C57BL/6 mice fed formononetin or 2-heptyl formononetin (C7F) (experiment 1) (n=23-25). Three-dimensional principal component analysis (3D-PCA) based on denaturation gradient gel electrophoresis data. Black circle: cholesterol, red square: formononetin, green square: C7F. The clustering was significant on the Y-axis and the Z-axis. On the Y-axis C7F fed mice clustered significantly differently from mice fed cholesterol and the formononetin. Also the formononetin fed mice clustered significantly differently from mice fed cholesterol. On the Z-axis the C7F fed mice clustered borderline differently from mice fed cholesterol.
Supplementary Figure 2: Gene expression of phase I and II metabolites (experiment 1). Scatter plot of liver gene expression (n=23-25). Gene expression in A mice fed chow compared to formononetin, B chow compared to C7F and C formononetin compared to C7F.
Supplementary Figure 3: Body weight of C57BL/6 mice fed chow, cholesterol or cholesterol supplemented formononetin or 2-heptyl-formonetin (C7F) for five weeks (experiment 2) (n=8). Graph shows mean ± SEM.
Supplementary Figure 4: Total feed intake in C57BL/6 mice fed chow, cholesterol or cholesterol supplemented formononetin or 2-heptyl-formonetin (C7F) for five weeks (experiment 2) (n=8).
Graphs shows mean ± SEM.