Table 1: Body composition as shown by DXA scans of C57BL/6 mice before cholesterol feeding, after five weeks of initial cholesterol feeding and after three additional weeks where the mice were fed the cholesterol-enriched diet supplemented with either formononetin or 2-heptyl-formonetin (C7F) (Experiment  1). Data show mean ± SEM. Different letters (a, b, c) denote significant difference between the groups.

Fat percentageBody weight
(g)
Fat mass
(g)
Bone mineral concentration
(g)
Bone mineral density
(mg/cm3)

Before test period

After acclimatisation ( )19.3 ± 7.8a16.7 ± 2.0a3.2 ± 1.80.3 ± 0.1ab 640 ± 9a
After initial cholesterol feeding ( )13.7 ± 6.8b24.3 ± 1.3b3.2 ± 1.70.3 ± 0.1a802 ± 10b

After test period

Cholesterol ( )14.8 ± 8.2b28.0 ± 2.1c4.2 ± 2.6 0.4 ± 0.07b864 ± 6c
Cholesterol + formononetin ( )13.1 ± 9.6b27.8 ± 2.3abc3.6 ± 2.80.4 ± 0.11ab839 ± 9bc
Cholesterol + C7F ( )14.5 ± 5.5b26.1 ± 2.2d3.2 ± 1.70.4 ± 0.08ab825 ± 10bc