Effects of Sodium Octanoate on Innate Immune Response of Mammary Epithelial Cells during Staphylococcus aureus Internalization

Table 1

Primers used in this study.

Specificity		Primer sequence (5′-3′)	Fragment size (bp)	Annealing temperature (°C)	References
Tracheal antimicrobial peptide (TAP)	F; R	GCGCTCCTCTTCCTGGTCCTG; GCACGTTCTGACTGGGCATTGA	216	57	[10]
Bovine neutrophil β-defensin 5 (BNBD5)	F; R	GCCAGCATGAGGCTCCATC; TTGCCAGGGCACGAGATCG	143	55	[15]
Lingual antimicrobial peptide (LAP)	F; R	GCCAGCATGAGGCTCCATC; CTCCTGCAGCATTTTACTTGGG	194	54	[15]
Bovine neutrophil β-defensin 10 (BNBD10)	F; R	GCTCCATCACCTGCTCCTC; AGGTGCCAATCTGTCTCATGC	152	54	[11]
Bovine neutrophil β-defensin 4 (BNBD4)	F; R	GCCAGCATGAGGCTCCATC; CGTTTAAATTTTAGACGGTGT	278	54	[15]
Bovine β-defensin 1 (DEFB1)	F; R	CCATCACCTGCTCCTCACA; ACCTCCACCTGCAGCATT	185	54	[11]
Bovine psoriasin (S100A7)	F; R	GCAGCTCTCAGCTTGAGCAG; CCAGCAAGGACAGGAACTCAG	221	54	[11]
Tumor necrosis factor-alpha (TNF-α)	F; R	CCCCTGGAGATAACCTCCCA; CAGACGGGAGACAGGAGAGC	101	55.5	[16]
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	F; R	TCAACGGGAAGCTCACTGG; CCCCAGCATCGAAGGTAGA	237	56.9	[17]