Improvement of Hydrodynamics-Based Gene Transfer of Nonviral DNA Targeted to Murine Hepatocytes

Figure 3

PCR analysis of the transgene for the identification of the recombined form of pCRTEIL in the liver. Through HGD, the mice received an intravenous injection of pCRTEIL and pTR/NCre; pCRTEIL alone; pTR/NCre alone; or PBS(-) alone (Mock). One day after HGD, the livers (Liv), lungs (Lu) and kidneys (Kid) of the mice in each group were dissected to obtain the transgene. PCR was performed by using the primer set described previously in the Materials and Methods section.