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BioMed Research International
Volume 2013 (2013), Article ID 930281, 10 pages
http://dx.doi.org/10.1155/2013/930281
Research Article

Photo-Induced Antitumor Effect of 3,6-Bis(1-methyl-4-vinylpyridinium) Carbazole Diiodide

1Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 10002, Taiwan
2Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 40254, Taiwan
3Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan
4Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei 10002, Taiwan

Received 21 July 2012; Accepted 6 September 2012

Academic Editor: Tobias Kiesslich

Copyright © 2013 Ya-Shuan Chou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We have applied a fluorescent molecule 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (BMVC) for tumor targeting and treatment. In this study, we investigated the photo-induced antitumor effect of BMVC. In vitro cell line studies showed that BMVC significantly killed TC-1 tumor cells at light dose greater than 40 J/cm2. The fluorescence of BMVC in the tumor peaked at 3 hours and then gradually decreased to reach the control level after 24 hours. In vivo tumor treatment studies showed BMVC plus light irradiation (iPDT) significantly inhibited the tumor growth. At day 24 after tumor implantation, tumor volume was measured to be  mm3,  mm3,  mm3, and  mm3 in the iPDT, control, light-only, and BMVC-only groups, respectively. Immunohistochemistry studies showed the microvascular density was significantly lower in the iPDT group. Taken together, our results demonstrated that BMVC may be a potent tumor-specific photosensitizer (PS) for PDT.