Direct Biodiesel Production from Wet Microalgae Biomass of Chlorella pyrenoidosa through In Situ Transesterification

Figure 4

Effects of n-hexane volume on the biodiesel yield by in situ transesterification of wet Chlorella pyrenoidosa biomass. Reaction conditions: 100 mg dry weight equivalent wet biomass (oil content 47%, water content 90%) with 4 mL methanol and 0.5 M sulfuric acid at 120°C for 120 min. n-Hexane volume: 2, 4, 6, 8, and 10 mL.