Research Article

Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells

Figure 2

Flow cytometry analysis of the enrichment of cells positive for each marker before and after cryopreservation. The numbers of viable cells positive for each marker were determined by flow cytometric analysis and are expressed as a percentage of the total viable cells in each condition. VASA positive cells were fixed before staining. VASA positive cells were counted without information about which ones were viable or dead. This graph shows that the average percentage of cells positive for each marker is higher after cryopreservation of cells and tissue. Two-sample -test was used for statistical analysis and was considered significant. .
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