Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells

Table 1

Viability of cells before and after cryopreservation.

Patient	Viability of cells
	Fresh cells	Frozen cells	Frozen tissue
1	84.9%	38.1%	70.1%
2	91.9%	64.1%	81.8%
3	93.0%	53.8%	68.9%
4	90.6%	53.3%	75.9%
5	90.2%	52.8%	73.5%
Average	90.1%	52.4%^a	74.0%^b
SEM	1.3%	3.9%	2.2%

The viability of each cell population was determined by dividing the number of viable cells counted with the number of total cells counted (viable + dead). Two sample 𝑡 test was used for statistical analysis and 𝑃<0.05 was considered as significant. ^ab𝑃 = 0.0019.