Research Article

Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells

Table 4

Pros and cons of cryopreservation of testicular cells and tissue for clinical application.

Material to FreezeProsCons

Tissue(1) Requires less time and effort.
(2) Less expensive.
(3) Requires fewer equipment to keep validated.
(1) Cells have to be isolated first before transplantation or other procedure.
(2) No information about the sterility of the cell product can be acquired at the time of transplantation.
(3) No information about the cellular composition is collected.
(4) No quality control and stability test can be done.

Cells(1) A complete profile of the viability, sterility, and cellular composition has been collected before cryopreservation.
(2) Small samples of cell product can be used for quality control and stability testing during long-term storage.
(3) The cell product with the known quality can be safely stored and sent to the clinic prior transplantation.
(1) Requires more time and effort for cell dissociation.
(2) More expensive.
(3) Has to be done in a cGTP compliant cell processing facility.