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Figure 4: Detection of BPV-2 E5 DNA. M: 50 bp molecular marker (HyperLadder II Bioline); 1-2: urinary bladder samples from healthy buffaloes without BPV-2 E5 DNA; 3–10: some representative bladder tumors samples showing BPV-2 E5 DNA; 11: positive control (cloned BPV-2 DNA); 12: negative control (no DNA added). The arrow indicates the position of the 154 bp BPV-2 E5 PCR product.