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Figure 5: Assessment of BPV-2 E5 cDNA by reverse transcriptase polymerase chain reaction (RT-PCR). M: 50 bp molecular marker (HyperLadder II Bioline); 1-2: healthy urinary bladder samples; 3–10: bladder tumors samples; 11: positive control (cloned BPV-2 DNA); 12: negative control (no DNA added). The arrow indicates the position of the 154 bp BPV-2 E5 PCR product.