Table 1: List of E. faecium primers used in the study.

Housekeeping genesPrimer sequences (5′-3′)

adk Forward GAACCTCATTTTAATGGGG
Reverse TGATGTTGATAGCCAGACG
atpA Forward CGG TTC ATA CGG AAT GGC ACA
Reverse AAG TTC ACG ATA AGC CAC GG
ddl Forward GAG ACA TTG AAT ATG CCT TAT G
Reverse AAA AAG AAA TCG CAC CG
gyd Forward CAA ACT GCT TAG CTC CAA GG C
Reverse CAT TTC GTT GTC ATA CCA AGC
gdh Forward GGC GCA CTA AAA GAT ATG GT
Reverse CCA AGA TTG GGC AAC TTC GTC CCA
purK Forward CAGATTGGCACATTGAAAG
Reverse TTCATTCACATATAGCCCG
pstS Forward TTG AGC CAA GTC GAA GCT GGA
Reverse CGT GAT CAC GTT CTA CTT CC

Adk: adenylate kinase; atpA: ATP synthase, alpha subunit; ddl: D-alanine: D-alanine ligase; gyd: glyceraldehyde-3-phosphate dehydrogenase; gdh: glucose-6-phosphate dehydrogenase; purK: phosphoribosylaminoimidazole carboxylase ATPase subunit; pstS: phosphate ATP-binding cassette transporter.