High Genetic Diversity of Enterococcus faecium and Enterococcus faecalis Clinical Isolates by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing from a Hospital in Malaysia

Table 1

List of E. faecium primers used in the study.

Housekeeping genes	Primer sequences (5′-3′)
adk	Forward GAACCTCATTTTAATGGGG; Reverse TGATGTTGATAGCCAGACG
atpA	Forward CGG TTC ATA CGG AAT GGC ACA; Reverse AAG TTC ACG ATA AGC CAC GG
ddl	Forward GAG ACA TTG AAT ATG CCT TAT G; Reverse AAA AAG AAA TCG CAC CG
gyd	Forward CAA ACT GCT TAG CTC CAA GG C; Reverse CAT TTC GTT GTC ATA CCA AGC
gdh	Forward GGC GCA CTA AAA GAT ATG GT; Reverse CCA AGA TTG GGC AAC TTC GTC CCA
purK	Forward CAGATTGGCACATTGAAAG; Reverse TTCATTCACATATAGCCCG
pstS	Forward TTG AGC CAA GTC GAA GCT GGA; Reverse CGT GAT CAC GTT CTA CTT CC

Adk: adenylate kinase; atpA: ATP synthase, alpha subunit; ddl: D-alanine: D-alanine ligase; gyd: glyceraldehyde-3-phosphate dehydrogenase; gdh: glucose-6-phosphate dehydrogenase; purK: phosphoribosylaminoimidazole carboxylase ATPase subunit; pstS: phosphate ATP-binding cassette transporter.