Unmasking Snake Venom of Bothrops leucurus: Purification and Pharmacological and Structural Characterization of New PL Bleu TX-III
Figure 1
(a) Elution profile of Bothrops leucurus venom by RP-HPLC on a μ-Bondapack C18 column. *Fraction 5 (PLA2 Bleu TX-III) contained PLA2 and myotoxic activity. Insert: rechromatography on RP-HPLC of Bleu TX-III. (b) PLA2 activity of Bothrops leucurus venom, b/K49, b/D49 [8], and PLA2 Bleu TX-III. The results of all experiments are the mean ± SEM of three determinations (). (c) Mass determination of the native Bleu TX-III by Q-Tof Ultima API ESI/MS (TOF MS mode) mass spectrometry. Insert electrophoretic profile in Tricine SDS-PAGE. (d) Raw electrospray-positive mass spectrum, showing multicharged ions distributions of native Bleu TX-III.
