Unmasking Snake Venom of Bothrops leucurus: Purification and Pharmacological and Structural Characterization of New PL Bleu TX-III
Figure 4
(a) and (b) Time-course of the increments in plasma CK activity after intramuscular injection and intravenous of Bleu TX-III PLA2 (1, 5, and 20 μg). Controls were injected with 100 μL of PBS. At different times, blood was collected, and serum levels were measured. Values are means ± SEM of five mice at each time point. (c) Edema-forming activity of Bleu TX-III PLA2 (1, 5 and 20 μg). In mice. Induction of edema by Bleu TX-III PLA2, injected in the footpad of mice. At various time intervals the increase in footpad volume, as compared to controls, was expressed as percent edema. Each point represents the mean ± SEM of five animals. (d), (e), and (f) Cytokines levels in mice. The production of IL-1, IL-6, and TNF-α was assayed in plasma of Bleu TX-III PLA2. The experiments were performed in triplicate and analyzed statistically by ANOVA or Kruskal-Wallis tests and confirmed by Tukey and Tukey-type tests. Each point represents ±SEM of seven mice between the experimental groups and control group.
