941764.fig.003a
(a)
941764.fig.003b
(b)
941764.fig.003c
(c)
941764.fig.003d
(d)
941764.fig.003e
(e)
Figure 3: Expression of inflammatory cytokines IL-6, TNF-α, IL-10, IL-1β, and IL-12 in macrophages, at 10 and 30 mM glucose, by real-time PCR. Cells were plated at 2 × 106/well and treated with 10 nM NT or 1 μg/mL LPS or both, during 6 h. Total RNA was isolated as described in “Materials and Methods.” The relative gene expression is indicated as arbitrary units and was obtained after normalization with the HPRT gene. Results are presented as mean ± SEM of six to ten independent experiments. ; .