Depletion of Luminal Pyridine Nucleotides in the Endoplasmic Reticulum Activates Autophagy with the Involvement of mTOR Pathway
Figure 5
Time course of the effect of high concentration metyrapone on phosphorylation status of mTOR pathway proteins. HepG2 cells were treated with 100 μM metyrapone for 5–120 min at 37°C. Each sample was analysed by Western blot by immunoreacting with antibodies against mTOR/FRAP-P, mTOR/FRAP, 4EBP1-P, 4EBP1, p70S6K-P, and p70S6K, respectively. The relative density of phosphorylated FRAP/GAPDH was represented. As positive control of autophagy cells were treated with 100 nM rapamycin for 1 hour at 37°C.
