In Vitro Antiophidian Mechanisms of Hypericum brasiliense Choisy Standardized Extract: Quercetin-Dependent Neuroprotection
Figure 1
Neutralizing activity of H. brasiliense ethanolic plant extract (HBE) against Crotalic venom, crotoxin and crotamine at mouse phrenic nerve-diaphragm preparations. Panel (a) shows the inhibitory effect of HBE (10 and 100 μg/mL) against C. d. terrificus venom (10 μg/mL), crotoxin and crotamine. In Panel (b) effect of Quercetin (10 and 100 μg/mL) against Cdt (10 μg/mL), crotoxin (1 μg/mL), and crotamine (1 μg/mL). When HBE was applied alone in the organ-bath no alteration in the twitch-tension was observed. On both graphs control Tyrode solution lines show no alteration of normal nerve-muscle activity. The points on the graphs represent the mean ± S.E.M. of five experiments. On (b) note that quercetin mimicked the protective effect induced by HBE. HBE: Hypericum brasiliense standardized extract * compared to control Tyrode.
