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BioMed Research International
Volume 2013 (2013), Article ID 945638, 11 pages
http://dx.doi.org/10.1155/2013/945638
Research Article

The Conyza triloba Extracts with High Chlorophyll Content and Free Radical Scavenging Activity Had Anticancer Activity in Cell Lines

1Department of Biological Sciences and Department of Chemistry, Faculty of Science, King Faisal University, Al-Hofuf, Al-Ahsa 31982, Saudi Arabia
2Department of Zoology, Faculty of Science, University of Ain Shams, Abbassia, Cairo 11566, Egypt
3Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt

Received 4 March 2013; Revised 3 April 2013; Accepted 14 April 2013

Academic Editor: Elvira Gonzalez De Mejia

Copyright © 2013 Wael M. El-Sayed et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The discovery of anticancer agents paradigm has been shifted to natural resources to overcome the toxicity of many synthetic agents at early clinical stages. In the present study, the antimutagenic, anticancer, phytochemistry, and free radical scavenging activities of five extracts of Conyza triloba were investigated. Extracts II (water : methanol), III (methylene chloride), and IV (methylene chloride : methanol) had the highest chlorophyll content and the highest superoxide scavenging, and metal chelating activities comparable to that of trolox. They also showed DPPH scavenging activities better than that of α-tocopherol. Virtually all extracts exerted a strong (>40% reduction) antimutagenic activity against sodium azide and benzopyrene. Extracts II, III, and IV showed a remarkable growth inhibition profile with GI50 of 0.07–0.87 μg for Hepa1c1c7 and H4IIE1, A549, HT29, and PC3 cell lines and totally abated the growth of all cell lines, except for the breast cells, at 0.3–7.0 μg. The present study found a strong correlation between the chlorophyll content of Conyza extracts and their DDPH scavenging, metal chelating, and in vitro cytotoxic and cytostatic activities most probably through triggering apoptosis. This study could offer a platform for future studies and help selecting the vital features that identify the extract with potential anticancer activities.