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BioMed Research International
Volume 2013 (2013), Article ID 947687, 6 pages
http://dx.doi.org/10.1155/2013/947687
Research Article

Role of Omega-3 Polyunsaturated Fatty Acids in the Production of Prostaglandin E2 and Nitric Oxide during Experimental Murine Paracoccidioidomycosis

1Department of Agricultural Sciences-Food Science, State University of Maringa, Avenida Colombo 5.790, 87.020-900 Maringá, PR, Brazil
2Department of Basic Health Sciences, State University of Maringa, Avenida Colombo 5.790, 87.020-900 Maringá, PR, Brazil
3Department of Chemistry, State University of Maringa, Avenida Colombo 5.790, 87.020-900 Maringá, PR, Brazil

Received 18 April 2013; Revised 25 September 2013; Accepted 7 October 2013

Academic Editor: Yoshio Ishibashi

Copyright © 2013 S. C. Sargi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

There has recently been increased interest in the potential health effects of omega-3 polyunsaturated fatty acids on the immune system. Paracoccidioidomycosis is the most important endemic mycosis in Latin America. Macrophages have a fundamental role and act as first line of organism defense. The purpose of this study was to analyze the effect of n-3 fatty acids on the production of PGE2 and NO by mice infected with Pb18 and fed a diet enriched with LNA for 8 weeks. To study the effect of omega-3 fatty acids on macrophage activity during experimental paracoccidioidomycosis, mice were infected with Pb18 and fed a diet supplemented with LNA. PGE2 in the serum of animals was analyzed and NO in the supernatants of macrophages cultured and challenged in vitro with Pb18 was measured. Omega-3 fatty acids seemed to decrease the production of PGE2 in vivo in the infected group fed an LNA-supplemented diet during the 4th and 8th weeks of the experiment. At the same time, we observed an increase in synthesis of NO by peritoneal macrophages in this group. Omega-3 fatty acids thus appear to have an immunomodulatory effect in paracoccidioidomycosis.