Gene Gun Bombardment with DNA-Coated Golden Particles Enhanced the Protective Effect of a DNA Vaccine Based on Thioredoxin Glutathione Reductase of Schistosoma japonicum

Table 1

Protective efficacy induced by pVAX1/SjTGR in mice.

Groups	Worm reduction		Egg reduction
	Worm burden; (mean ± SE)	Percent reduction in worm burden (%)^a	Liver egg per gram (EPG) (mean ± SE)	Percent reduction in liver egg count (%)^b
Trail 1
pVAX1/SjTGR/g.g. (𝑛=10)^c	15.6±8.86 *	27.83	31591.3±14647.42 *	40.38
pVAX1/g.g. (𝑛=10)	23.3±6.85	−8.03	42995.7±11730.94	18.86
pVAX1/SjTGR/i.m. (𝑛=10)	24.6±12.18	−14.12	25558.7±15171.82 *	51.77
pVAX1/i.m. (𝑛=10)	23.7±5	−8.09	57686.6±20951.58	−8.86
PBS (𝑛=10)	21.6±3.94	—	52989.6±19448.42	—
Trail 2
pVAX1/SjTGR/g.g. (𝑛=10)	12.6±7.17 *	38.83	33326.5±6875.44 *	44.51
pVAX1/g.g. (𝑛=10)	21.6±8.67	−4.85	65934.8±16702.02	−9.79
PBS (𝑛=10)	20.6±4.90	—	60055.1±19211.27	—

Differences were significant at 𝑃<0.05 (*); data was presented in 95% confidence interval.
^aPercent reduction was determined using total worms in immunized group compared to PBS group.
^bPercent reduction was calculated using liver egg burden in immunized group compared to PBS group.
^cThe number of animals in each group when perfusion.