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BioMed Research International
Volume 2013 (2013), Article ID 956878, 9 pages
http://dx.doi.org/10.1155/2013/956878
Research Article

Plasma Levels of Soluble HLA-E and HLA-F at Diagnosis May Predict Overall Survival of Neuroblastoma Patients

1Laboratorio di Oncologia, Istituto Giannina Gaslini, Via G. Gaslini 5, 16148 Genova, Italy
2Laboratorio di Analisi, Istituto Giannina Gaslini, Via G. Gaslini 5, 16148 Genova, Italy
3Oncologia Clinica, Istituto Giannina Gaslini, Via G. Gaslini 5, 16148 Genova, Italy
4Oncoematologia, Ospedale Pausillipon, Via Mario Fiore 6, 80123 Napoli, Italy
5Epidemiologia, Biostatistica e Comitati, Istituto Giannina Gaslini, Via G. Gaslini 5, 16148 Genova, Italy

Received 29 July 2013; Accepted 24 September 2013

Academic Editor: Maria Lina Tornesello

Copyright © 2013 Fabio Morandi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study was to identify the plasma/serum biomarkers that are able to predict overall survival (OS) of neuroblastoma (NB) patients. Concentration of soluble (s) biomarkers was evaluated in plasma (sHLA-E, sHLA-F, chromogranin, and B7H3) or serum (calprotectin) samples from NB patients or healthy children. The levels of biomarkers that were significantly higher in NB patients were then analyzed considering localized or metastatic subsets. Finally, biomarkers that were significantly different in these two subsets were correlated with patient’s outcome. With the exception of B7H3, levels of all molecules were significantly higher in NB patients than those in controls. However, only chromogranin, sHLA-E, and sHLA-F levels were different between patients with metastatic and localized tumors. sHLA-E and -F levels correlated with each other but not chromogranin. Chromogranin levels correlated with different event-free survival (EFS), whereas sHLA-E and -F levels also correlated with different OS. Association with OS was also detected considering only patients with metastatic disease. In conclusion, low levels of sHLA-E and -F significantly associated with worse EFS/OS in the whole cohort of NB patients and in patients with metastatic NB. Thus, these molecules deserve to be tested in prospective studies to evaluate their predictive power for high-risk NB patients.