Anti-Inflammatory Activity of Bioaccessible Fraction from Eryngium foetidum Leaves
Figure 3
The effect of the bioaccessible fraction of EF generated in the presence and absence of bile extract during simulated digestion on secretion of IL-8 and MCP-1 and the intracellular ROS activity in IL-1β activated Caco-2 cells. In differentiated cultures, Caco-2 cells were incubated for 4h with diluted (1 : 3) bioaccessible fraction of digested EF generated with or without bile extract prior to the addition of IL-1β. Cell culture medium was collected after 20 h to quantify IL-8 (a) and MCP-1 (b). After washing with PBS, monolayers were incubated with DCF-DA before lysing with Triton X-100. Supernatant was collected to measure fluorescent as a marker for intracellular ROS as described in method (c). Results are mean SD for the three independent experiments. Means without a common letter above the error bar differ significantly ().
