BioMed Research International / 2013 / Article / Tab 2 / Review Article
Microbial Diversity in the Era of Omic Technologies Table 2 Technical specifications of Next Generation Sequencing platforms.
Platform 454 Illumina Life technologies ABI/SOLID Helicos biosciences heliscope Ion torrent Pacific biosciences Year of availability 2005 2006 2006 2007 2010 2010 Sequencing length 200–700 bp Up to 150 bp 35–50 bp 25–55 bp ~200 bp 1500 bp Sequence yield per run 700 Mb 2–600 Gba 120 Gb 35 Gb 20–50 Mb on 314 chip 100–200 Mb on 316 chip Gb on 318 chip 100 Mb Run time 23 h 27 h–11 days 7-8 days 3–6 days 2 h 2 h Technology emPCR, pyrosequencing Polonies, cleavable dye terminators emPCR, ligation with cleavable dye terminators
True Single Molecule Sequencing (tSMS)Single base, reversible dye terminator extension reactions emPCR, H+ detection Single Molecule Real Time (SMRT) sequencing dyes that are phospholinked to the nucleotide, very sensitive fluorescent detection in zero mode waveguides
2 Gb for the MiSeq and 600 Gb for the HiSeq2000.