Table 2: The main characteristics of IL-1 gene and techniques used for screening.

IL-1αIL-1β

Polymorphism typeSingle base C/TSingle base C/T
Polymorphism site−889−511
PCR primers
 Upstream5-GGGGGCTTCACTATGTTGCCCA
CACTGGACTAA-3
5-TGGCATTGATCTGGTTCATC-3
 Downstream5-GAAGGCATGGATTTTTACATAT
GAGCCTTCCATG-3
5-GTTTAGGAATCTTCCCACTT-3
PCR condition
 Denaturation94°C94°C
 Annealing57°C55°C
 Extension72°C72°C
 Cycles ( )3535
 DigestionNCOIAvaI
 Fragment size (pb)295304