- About this Journal ·
- Abstracting and Indexing ·
- Aims and Scope ·
- Annual Issues ·
- Article Processing Charges ·
- Articles in Press ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 963525, 10 pages
Waterlogging Tolerance of Crops: Breeding, Mechanism of Tolerance, Molecular Approaches, and Future Prospects
1Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
3Bioscience and Agrotechnology Division, Malaysian Nuclear Agency, Bangi, 43000 Kajang, Selangor, Malaysia
4Rice and Industrial Crop Research Centre, Malaysian Agriculture Research and Development Institute (MARDI), Locked Bag No 203, Kepala Batas Post Office, 13200 Seberang Perai, Pulau Pinang, Malaysia
5Plant Pathology Division, Bangladesh Rice Research Institute (BRRI), Gazipur, Dhaka, Bangladesh
Received 20 October 2012; Accepted 14 November 2012
Academic Editor: Andrei Surguchov
Copyright © 2013 F. Ahmed et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Submergence or flood is one of the major harmful abiotic stresses in the low-lying countries and crop losses due to waterlogging are considerably high. Plant breeding techniques, conventional or genetic engineering, might be an effective and economic way of developing crops to grow successfully in waterlogged condition. Marker assisted selection (MAS) is a new and more effective approach which can identify genomic regions of crops under stress, which could not be done previously. The discovery of comprehensive molecular linkage maps enables us to do the pyramiding of desirable traits to improve in submergence tolerance through MAS. However, because of genetic and environmental interaction, too many genes encoding a trait, and using undesirable populations the mapping of QTL was hampered to ensure proper growth and yield under waterlogged conditions Steady advances in the field of genomics and proteomics over the years will be helpful to increase the breeding programs which will help to accomplish a significant progress in the field crop variety development and also improvement in near future. Waterlogging response of soybean and major cereal crops, as rice, wheat, barley, and maize and discovery of QTL related with tolerance of waterlogging, development of resistant variety, and, in addition, future prospects have also been discussed.
In the world of increasing food demand scientists are trying to produce more crop in the barren farmland and in unfavorable condition. Usually in those areas water, soil condition, and temperature are not favourable for agricultural production. Waterlogging is one of the most hazardous natural occurrences, which can also be called as flood, submergence, soil saturation, anoxia, and hypoxia, which are generally used to describe waterlogging conditions depending upon the moisture or water level on the field. Generally, two types of flooding are present in the field: (1) waterlogging, in which root and some portion of the shoot goes under water, and (2) complete submergence, where the whole plant goes under water.
Landraces cultivated by local farmers include varieties adapted to waterlogging or complete submergence which could be used as a source of genetic material for improving the tolerance of rice to some environmental stresses like waterlogging. Almost a total of over 22 million hectares of lowland rice cultivable area worldwide are vulnerable to flash flooding, representing a total of almost 18% of the global supply . 10 million hectares of land in India and Bangladesh are regularly affected by flood in monsoon season regularly . To avoid such damage, farmers traditionally cultivate flood-adopted lowlands and landraces which can withstand complete submergence or flooding for 10 days or more and can resume its growth after desubmergence . Usually these landraces have total yield not more than 2 t/ha, in contrast to semidwarf varieties which have yield of 6–8 t/ha. Regrettably, the “mega varieties” which are grown widely in different parts of Asia are prone to flooding and dies within one week. Plant breeders identified this problem in the 1970s, while trying to increase production in the low-lying areas by introducing submergence tolerance traits to high-yielding varieties which are popular to local farmer .
Lowland rice varieties are usually cultivated in fields with standing water of 5–25 cm, usually vulnerable to seasonal flash floods of about 50 cm or more water level which can completely submerge entire field of crops. Frequently, stagnant flooding also occurs due to heavy rainfall with flash flood and deepwater flood. In case of flash flood plant restricts its growth when under water and after removal of water growth is restored. In deepwater flood, most of the rice variety show unique capacity of elongation of leaves and some part of stem (internode) which is entrapped under water. After flood this elongated tall plant easily lodges when flood water moves away. In case of deep water flood, underwater elongation could cause death of the plant within few days by exhausting reserve energy of the plant.
In case of barley, waterlogging causes chlorophyll, protein, and degradation of RNA, and also reduces the concentration of nutrients like nitrogen, phosphorus, metal ions, and minerals in shoot. After the onset of flood leaf chlorosis starts [5–7], root and shoot growth was also affected which results in reduction of accumulation of dry matter and also finally in yield [8–11]. Estimated 20–25% yield loss can happen depending upon plant damage; it may be exceed 50% due to waterlogging .
Maize is also susceptible to waterlogging which causes loss of yield in tropical and subtropical region. Fifteen percent of all maize growing areas of South-East Asia face waterlogging problem, which may lead to yield loss of a range about 25–30% annually .
Damages of soybean because of waterlogging are chlorosis, necrosis, stunting, defoliation, reduced nitrogen fixation, and plant death which causes yield loss [14–18] and all of these symptoms occur at various vegetative and reproductive stages of the plant [16, 18–21]. Only 2 days of flooding can cause 18% of yield loss at late vegetative stage while it may exceed to 26% if flooding occurs at early reproductive stage of soybean . Flooding regularly affect soybean growth and grain yield around the world including USA [21, 22].
Waterlogging is a major problem for wheat cultivation around the world and in USA where around 12% of cultivated soil affected by excess water . About 39-40% yield loss is recorded under waterlogging condition [24, 25]. Researchers suggested that it is a combining effect of reduced kernel and tiller numbers  which is responsible for reducing yield of wheat in waterlogging.
2. Mechanism of Waterlogging Tolerance
Waterlogging is a serious problem in low-laying rainfed areas. Lack of oxygen supply for the plant is the main reason of damage in waterlogging condition, which hampers nutrient and water uptake, as a reason the plant shows wilting. In oxygen-deprived condition plants shift its metabolism to anaerobic from aerobic mode. Plants which can withstand waterlogging condition have mechanisms such as increased availability of soluble sugar, aerenchyma formation, greater activity of glycolytic pathway and fermentation enzymes, and involvement of antioxidant defense mechanism to cope with the oxidative stress induced by waterlogging. Ethylene plays an important role in change of mechanisms of plants in deficiency of oxygen. It was also reported that ethylene induces the genes of enzymes associated with aerenchyma formation, glycolysis and fermentation pathway .
2.1. Hypoxia and Anoxia
Roots of the plants are usually in contact with oxygen at a partial pressure which is equivalent to the gaseous atmosphere. Hypoxia, reduction of oxygen below optimum level, is the most common form of stress which occurs during partial submergence of plant due to short-term flooding where the root goes under water and shoots remain in the atmosphere. Anoxia, another kind of water stress where plant goes under water completely, hence complete absence of oxygen as a result of long-term flooding. Microbial flora of the soil may change by long time waterlogging which works in favour of anaerobic microorganisms that use, as alternative, electron acceptors to oxygen. In such condition soil tends to accumulate nitrite as it tends to accumulate more reduced and phytotoxic forms of mineral ions (from nitrate) and ferrous ions (from ferric) and very few number of plants are naturally adapted to grow in this kind of soil .
2.2. Ethylene Production and Flooding
Accumulation of ethylene in waterlogged soil and plants occurs at concentration of 10 cm3 dm−3 . This accumulation of ethylene occurs in mainly two ways, firstly ethylene diffusion rate from root to water is 10 times slower than in air  and, ethylene synthesis is increased in the hypoxic root system and in the aerobic shoot . At first ethylene may be released to the internal channels of aerenchyma and then diffused to the root area. In roots high amount of ACC (1-amino cyclopropane 1-carboxylic acid) synthesis is occurred, which is a precursor of ethylene . ACC to ethylene conversion cannot happen without oxygen; as a result this reaction is blocked in the aerobic root system . Formation steps of ACC do not require oxygen, furthermore, accumulation of ACC rather increased in aerobic condition . As a result the amount of ACC is increased to the shoot. Highest amount of ACC accumulation occurs in the lowest portions of the stem and ethylene is released in the presence of oxygen. In partial submergence rice undergoes through a special pathway initiated by the entrapment of ethylene in the plant cell. As a result ethylene encourages degradation of abscisic acid (ABA) and increases the content of gibberellic acid (GA) and their downstream effects [33, 34]. Remarkably, the rates of stem elongation in deepwater rice varieties may be up to 25 cm/day. This phenomenon of deepwater rice is mainly controlled by three QTLs. Of these, the SNORKEL QTL on chromosome 12 encodes two ethylene responsive factor (ERF) DNA binding proteins, SNORKEL1 (SK1), and SNORKEL2 (SK2), which were absent to nondeepwater rice accessions .
2.3. Role of Ethylene in Aerenchyma Formation
Formation of aerenchyma is initiated by ethylene, which is one of many adaptive features of plants at submergence to avoid anaerobiosis by increasing the availability of oxygen. The soft tissues with large intercellular spaces to render low-resistance internal pathways for the exchange of gases between aerobic shoot to the anaerobic root are called Aerenchyma . This feature has been reported in many species like, Trifolium subterraneum , wheat , rice , barley , maize , soybean , Vigna luteola, and Carex spp. [41, 42]. Oxygen leaks out into the roots and surrounding soil through the aerenchyma. As a result, a small oxygenated soil environment formed which might create aerobic environment to microorganisms and prevent the development of potentially toxic soil components such as oxides of Iron, Cu, and Mn . Synthesis of ethylene was highly increased in roots under hypoxia condition, simultaneously with the formation of aerenchyma .
2.4. Role of Ethylene Accumulation in Adventitious Roots Formation
Portion of the plant stem in flooded condition produces adventitious roots and grows horizontally (diageotropism). This may be the new roots which replace old root systems . Because of the position of the new roots is close to water surface and they are connected to the stem, close to the formatted aerenchyma, they have more access to oxygen than the old root system. Large air space between these roots enables diffusion of gas between roots and shoots. Adventitious root primordia of deep water rice initiates as a normal plant development but the formation is initiated by the death of nodal epidermal cell covering the tip of primordia which occurred as a result of flood-induced ethylene development . This helps adventitious root development and prevents injury to the growing root.
3. Conventional Breeding for Tolerance
A good number of researches have been done to find out the morphological, physiological, anatomical, molecular, biochemical response of plant to anoxia and hypoxia for at least two decades [47–51]. Lack of oxygen has been suggested as main difficulty for plant , during the time and after the flooding plant may be affected by root rot disease which cause reduction of growth and loss of yield , also includes nitrogen deficiency of plant , or nutrient imbalance [54–56].
Crops in different low-laying areas were subjected to flooding for various periods. Few landraces and traditional varieties of rice have the mechanism to withstand flooding which was first recognized in 1950s and screened in 1970s. Indian accessions FR13A and FR43B and some Sri-Lankan varieties like, Kurkaruppan, Goda Heenati, and Thavalu have been known for their tolerance against complete submergence while FR13A was found to be 100% tolerant for 7 days of complete submergence of 10 days old seedlings [4, 57, 58]. Because of some poor agronomic characteristics like, photoperiod sensitivity, low yields and poor quality of grain, and tallness, FR13A needs to be crossed to produce submergence tolerant variety as a donor for submergence. Effort of producing such a variety started in 1980s [1, 59, 60] and in mid 1990s a submergence tolerant IR49830- 7-1-2-2 of FR13A-derived breeding line was produced and the submergence tolerant gene was introduced to high yielding short-to-intermediate statured lines [61, 62]. It was found that submergence tolerance was controlled by a single or few major genes with some minor genes with smaller effect .
However, because of their lodging-proneness and susceptibility to insect, pests, and disease these traditional-submergence tolerant varieties are not suitable for large scale production. For this reason IRRI tried to develop submergence tolerant variety with desirable characteristics. “IR 49830-7-2-2” is such a line which is high yielding and resistant to pest and disease and has been used as donor parent; India developed “Sudhir” which is derived from “FR13A” × “Biraj” crosses . Despite the above achievements, till date no desirable submergence tolerant variety with high yield has been developed.
Other major attempts include development of two population of DHL by IRRI using double haploid lines (DHL) by using cross between submergence tolerant and intolerant cultivars . Intermediate height high-yielding rice varieties like “Jagannath” (OUAT) and “Pankaj” (IRRI) in India in 1969 and “Mahsuri” in Malaysia in 1971 was a landmark in lowland rice breeding. In early 80’s two varieties “Savitri” and IR42 developed by Central Rice Research Institute (CRRI) and IRRI, respectively, proposed as high yielding and resistant to insect, pests, and diseases but not as tolerant to submergence as FR13A. Nowadays, scientists are using these varieties as a base material for the development of submergence tolerant variety.
Nowadays accumulated CO2 in the root zone of soybean plant has been considered as the main reason of injuries of plant. Breeding for flooding tolerant soybean cultivar aims at developing a variety which can give maximum grain yield from a flooded field where as other attributes such as plant height, root and shoot biomass, leaf colour, and so forth are also considered as major determinants . Application of that knowledge to produce flooding tolerant soybean variety is not yet successful which may be very useful for growers. Genetic viability for soybean flood tolerance has been discovered , marker assisted selection (MAS) has been successfully used to breed crops with genetically improved QTLs .
Various species of wheat were studied under continuous flooding conditions to determine difference of growth and yield . Waterlogging tolerant cultivars of wheat were identified  which unlocks the potential of waterlogging tolerant variety research of wheat. Presence of Adh gene in wheat which is also found in barley and rice are associated with waterlogging tolerance to ensure the presence of tolerance mechanism in wheat. All possible crosses between waterlogging tolerant cultivars of spring wheat were made and suggested that waterlogging is controlled by a few number of genes . Another experiment was conducted to find out the narrow-sense of heritability for grain yield and yield components of 80 families of soft red winter wheat population of in waterlogging condition to provide selection criteria for further breeding. The plant was kept on five week of waterlogging and REML (Restricted Maximum Likelihood) was used to find out the genetic variance components. Kernel weight was found to be the highest heritability (0.47) along with chlorophyll content (0.37), and tiller number (0.31) and the lowest heritability were grain yield () attributes. Kernel weight and grain yield was found to be highly correlated. Selection for a relatively highly heritable trait, as kernel weight, would be an effective way to improve waterlogging tolerance in early generations, as grain. Total yield increase of 17% could be done by selecting on the basis of the index: grain yield-kernel weight-tiller number .
4. Marker Assisted Breeding for Submergence Tolerance
Since in mid 1990s major genes responsible for waterlogging tolerance have been identified (Table 1) and it become easy for the researcher to concentrate on modification or use of those genes to develop new waterlogging tolerant crop varieties like rice, wheat, maize, soybean, barley, and so forth. In the case of rice introgression of Sub1 gene to specific varieties by marker assisted backcrossing (MAB) for various land types and choice of the farmer and addition of new varieties through genetic engineering became possible.
4.1. Identification of QTL Associated with Waterlogging Tolerance
Genetic control of submergence tolerance was unknown until mid-1990s. It was suggested as a quantitative trait [1, 59, 74, 75]. Sub1 the major QTL responsible for submergence tolerance was found out by molecular mapping on chromosome 9, which contributes almost 70% of phenotypic variation in tolerance . Furthermore, it was also suggested that the major chromosome 9 along with other minor QTL is not responsible for more than 30% of the submergence tolerance of rice [77–79]. Sub1 was mapped to a 0.16-cM region with circa 3,000 F2 progeny on 9th Chromosome  and using 4,022 individuals a fine-scale physical mapping of Sub1 also done, which further specified the position of locus 0.075 cM  using two different types of varieties, the resistant indica Teqing and tolerant FR13A derivative IR40931-26.
Detail analysis of ERFs related to Sub1 locus indicates the duplication of Sub1B leads to the development of Sub1A which may happen after the indica rice domestication . Based on variations of nucleotide in the protein coding region the Sub1A is divided in two allelic forms, for example, Sub1A-1 and Sub1A-2 in submergence tolerant and intolerant accessions of indica and aus lines . FR13A consists of Sub1A-1 which is only found in tolerant varieties, on the other hand, Sub1A-2 allele could be found in nontolerant indica accessions. Both of the allels encode identical proteins. The difference is, intolerant allele contains Pro186 and, in the tolerant allele it is Ser186. In case of expression, Sub1A-1 promotes rapid, prolonged, and pronounced transcript accumulation in leaves of 2- to 4-weeks-old plants in case of submerged condition; on the other hand Sub1A-2 promotes a lower level of transcript induction [69, 82]. Detailed analysis of different rice accessions with different Sub1 haplotype showed that submergence tolerance is associated with variable levels of Sub1A transcript in internodes and nodes at the heading stage of the plant .
For the identification of QTL associated with waterlogging tolerance of barley different parameters was set like leaf chlorosis, plant survival rate, and biomass reduction. Then compare the QTLs identified in two seasons in two different populations using a composite map prepared by the analysis of different molecular markers like SSRs, RFLP, and DArT (Diversity Array Technology). In two barley double haploids (DH) population twenty QTLs associated with waterlogging tolerance was identified and they were validated by different replication of experiments or by different location trials. It was suggested that most of the QTLs were associated with leaf chlorosis and plant survival while some of them affected multiple waterlogging related traits.Such as QTL, Qwt4-1 contributed to overcome different waterlogging related stress like leaf chlorosis and reduction of plant biomass .
Two maize inbreed lines were crossed for the purpose of determining waterlogging tolerance, that is, “HZ32” (highly waterlogging-tolerant) and “K12” (highly waterlogging sensitive) [84, 85] were crossed for developing mapping population. In the maize growing season of years 2004 and 2005, two experiments were conducted, namely, EXP.1 and EXP.2, respectively, with the same families along with parents and hybrid with a treatments of controlled (without flooding) and waterlogging. One hundred seventeen SSR markers with average space of 11.5 cM were used for the preparation of population genotyping which was a base-map of 1710.5 cM length. By the use of composite interval mapping (CIM), QTL associated with different plant characters like, root dry weight, root length, plant dry weight, plant height, shoot dry weight, and waterlogging tolerance coefficient were identified in both the experiments, EXP.1(2004) and EXP.2 (2005) in control and waterlogging condition, respectively. In EXP.1, 25 and in EXP.2, 34 QTL were identified where the effects of discovered QTLs were moderate with a range of 3.9–37.3%. Some major QTLs were identified in two chromosomes 4 and 9 and in both experiments associated with root dry weight, shoot dry weight, total dry weight, plant height, and their waterlogging tolerance coefficient. Chromosome 1, 2, 3, 6, 7, and 10 was also identified  as secondary QTLs associated with tolerance.
To determine the QTL associated with waterlogging tolerance of soybean, two hundred eight lines of two recombinant inbred (RI) populations, “Archer” × “Minsoy” and “Archer” × “Noir I”, were put into two different experimental setup, that is, one with control (without waterlogging) and other with waterlogged condition, and the plants of waterlogged setup were put into two weeks of flooding at early flowering stage. A single QTL was identified which was responsible for better plant growth and grain yields, 11–18% and 47–180%, respectively, in waterlogging environment linked to marker sat_064 from the parent Archer . In another experiment two populations, that is, A5403 × Archer (Population 1) and P9641 × Archer (Population 2) were used to identify QTLs associated in waterlogging tolerance where 103 and 67 RI lines, respectively, were used for mapping population. The performance of both the populations was tested on the flooded field setup and significant variations were observed, however, no transgressive tolerant segregants were found in both populations. Whole sample was divided into sensitive and tolerant bulk for the purpose of further molecular study.
In molecular genetics studies, two methods, named, SMA (single marker analysis) and CIM (composite interval mapping), were used for the identification of the QTL associated with waterlogging tolerance. Out of 912 SSR markers, 17 markers (population 1) and 15 markers (in population 2) were found associated with waterlogging tolerance in SMA. Most of the markers were closely related with Rps gene or QTL responsible for the resistance to Phytophthora (Phytophthora sojae M. J. Kaufmann and J. W. Gerdemann). In CIM five markers, that is, Satt59, Satt160, Satt269, Satt252, and Satt485 were found associated with waterlogging tolerance  in both populations. This analysis also suggested that most of the genes come from the parent Archer.
4.2. Manipulation of QTL for Developing Waterlogging Tolerant Variety
Flooding is a natural calamity that destroys production of rice throughout the world especially in low-laying areas, but all economically important varieties are not tolerant to flooding. Identification of the SUB1 QTL enables the scientist to introgress this gene by MAB into the popular high-yielding varieties [86, 87]. Mapping of the Sub1 allele reveals SNP (single-nucleotide polymorphisms) within Sub1A and Sub1C, which is useful for the breeders for molecular markers and in precision breeding [88, 89]. By the use of MAB, Sub1A has been already inserted to modern varieties of different countries; BR11, Swarna, Samba Mahsuri IR64, CR1009 and Thadokkam 1 (TDK1) are some of the examples of those varieties . SSR markers, which were polymorphic between two parents, were generally used for the background conformation or recurrent parent genome conformation also well combined with the Sub1 region originated from FR13A on chromosome 9. Normally newly developed Sub1 lines shows more significant waterlogging tolerance compared to parents [88, 90] which is find out by evaluation. These studies show the opportunities of the insertion of the Sub1 region from developed tolerant variety through MAB to produce tolerant varieties with a diverse genetic background. Furthermore, the effect of Sub1 on other agronomical characteristics of plant such as, grain quality, growth, maturation, and grain production was determined in IR64 Sub1, Swarna Sub1, and Samba Mahsuri Sub1. Further research of the above mentioned submergence tolerant varieties and their original parents revealed that insertion of Sub1 gene does not alter field performances including quality and yield of grain under normal growth condition [83, 89, 91].
Complete waterlogging of susceptible varieties at the different growing stage considerably reduces the yield attributes like, number of panicles, total number of grains per panicle, and grain filling percentage of the plant and also flowering and maturity may be delayed, resulting in a remarkable decline in yield. On the other hand, a Sub1 rice variety minimizes the total yield reduction by flood and produces more yield than the intolerant varieties at submerged condition .
One of the main advantages of using this approach is that the newly developed Sub1 varieties retain almost all agronomical characteristics of the recurrent parent, especially in terms of yield and quality. Sub1 varieties produced from FR13A-derived varieties have almost the same yield, agronomical, and grain quality characteristics as recurrent parent varieties when grown under regular condition, but, when subjected to flooding for 1 to 2 weeks, Sub1 varieties showed a remarkable advantages in terms of yield than the susceptible ones [83, 90]. Mega varieties which has Sub1 gene, can be adopted by the farmer easily, in addition, these new varieties can replace the traditional landraces with low yielding, currently which has been used by the farmers in flood prone areas.
Some tolerant lines were evaluated and adopted in low-lying areas in more than ten countries in South and Southeast Asia, in field trials as the preferences of the farmers . The good yield performance of newly developed varieties, showing the better performance of Sub1 against flooding or submergence of rice. Those performances of the newly developed varieties have influenced a lot of rice improvement programs in Asia and Southeast Asia to perform rapid seed multiplication and dissemination schemes.
5. Improvement of Flooding Tolerance in Rice through Plant Genetic Engineering Techniques
Current molecular biology and biotechnology methods for rice research have been improved to such level that rice has been considered as a model crop in cereal breeding. Transformation of gene of rice was firstly done by protoplast-based method through using microprojectile gun, and with the advance of technology with time Agrobacterium mediated gene transfer of rice is also available. The regulatory sequences of controlling expression of transgenes in rice are available now, so as a result, the transgene can be either normally expressed or expressed in response to a specific stimulus or artificially (including induced anaerobic stress, through the use of promoters from genes which strongly respond to waterlogging). Transgene rice with specific attributes like, tolerance to virus, insects. Pests, salt, low temperature, and flood can be produced . Identification/isolation/cloning of associated genes with waterlogging stress considered as limiting factor , as a result scientists focused on carbohydrate metabolism because reduced O2 supply hampers normal respiration resulting in decrease in ATP synthesis. It has been reported that, respiration pathway switch to fermentation pathway from oxidation during oxygen-deprived (anaerobiosis) condition. The inclusion of ethanolic fermentation pathway is considered to be an important component of responses which are elicited in rice (and other plants) against flooding stress [93, 94]. Ethanolic fermentation, that is, pyruvate to ethanol is a relatively simpler process involving two enzymes, that is, PDC or pyruvate decarboxylase and ADH or alcohol dehydrogenase. Availability of cloned pdc and adh normally attracts the interest of molecular biologists to use these for transgenic experiments. Researchers at CISRO, Australia are trying to alter the different levels of PDC and ADH in rice and as a result 3 different pdc genes in rice, that is, pdc1, pdc2, and pdc3 have already been cloned and sequenced. pdc1 cDNA has been subcloned at the 3′ end of three different promoters that is, CaMV 35S, actin 1 and anoxia-induced 6X ARE promoter, which is a synthetic promoter, in both sense and antisense orientations and these plasmid constructs have been introduced into rice to produce a large number of transgenes [95–97]. One of the above gene constructs (actin 1-pdc1, sense) has been employed at IRRI for the production of transgenic rice with enhanced metabolic capacity under anaerobiosis conferring submergence tolerance . In the study, tillers of transformed plants show higher pdc activities and ethanol production than untransformed plants and consequently ethanol production of tillers of transgenic plants was positively correlated with survival after submergence. Scientist of CISRO also made considering progress to change in the ADH level in rice . However, no attempts directed at making transgenes by using those have been taken.
6. Conclusions and Future Prospects
Combination of both MAS and traditional field selection could increase the efficiency of barley breeding programs. Now, it is suggested that diversity array technology (DArT) is most useful for genome profiling . DArT markers can be used for the analysis of waterlogging tolerant genes or QTLs in grass families, and in future researches it may be possible to make comparison of a DNA sequence to the genome sequence of rice and other species [101, 102].
Physiologies of rice plant under submerged condition have been studied intensively since the work of Yamada . The damage caused by waterlogged condition may be associated to the associated action by water in gas exchange and illumination. Reaction of the plats to avoid such damage caused by impeded gas exchange includes an increase of leaf elongation, stopping photosynthesis, and leaf senescence. These actions may be taken by the plant to decrease energy demand of the plant to maintain cell integrity in important tissues like meristematic region of leaf bases, root tips, and stem. Survival mechanism of rice plants against submergence became clearer. It is proposed that tolerance of a plant is permitted by minimizing the imbalance between production of energy and its usage. This is promoted by a suppression of the high rates of leaf elongation that submergence normally does. Furthermore, reduction of leaf senescence during submergence has been implicated. Elongation of leaves in waterlogged condition and senescence could be implicated by ethylene which is trapped within the plant cell because of its diffusive escape which is more delayed by water then air. These ethylene-induced effects are suppressed in tolerant lines. Researchers also find out that presence of Sub1 locus on chromosome 9 of rice which have act upon senescence and leaf elongation, effects of injury, and survival. This effect is dominant, that is, effective in the heterozygote carrying only one FR13A-derived segment  and may affect ethylene responsiveness of the plant. It is one of the major challenges for scientists to modify the effects of environmental stress [97, 104]. Tightly linked DNA markers gives opportunities for transferring the submergence tolerance characteristics from FR13A or other lines into economically important cultivars. Such markers are known to be carried over when tolerance is introduced into a desirable agronomical background by conventional breeding and progeny phenotyping . Identification of other mechanisms of tolerance derived from resilient cultivars other than FR13A (e.g., Goda Heenati and Vaidehi) remains unknown and represent a promising additional resource for breeders.
REML method could be very useful in wheat waterlogging tolerance breeding. Breeding programme selecting combination of different criteria including grain yield, kernel weight, and so forth instead of only one character could be more useful to develop high-yielding variety. The total gain from each selection should be confirmed prior to taking steps which lead to the development of new variety .
First work about submergence tolerance of maize seedling was done by Mano et al. . Several OTLs associated with waterlogging tolerance with genome-wide significance has been identified which may be useful to clarify the understanding of maize waterlogging tolerance mechanism . Use of different evaluation method, or population, or combination, of both may enables the researchers to detailed understanding of tolerance. Already identified QTLs should be mapped precisely before inserting prior to develop tolerant variety.
Genomic regions responsible for waterlogging tolerance of soybean can be helpful for MAS to develop high-yielding flooding tolerance cultivars. It may be possible to combine multiple favourable alleles into one cultivar. Variety selection to use as rotation of soybean and in low-yielding areas vulnerable to waterlogging conditions will be benefited by significant QTLs tolerance information. Further study should be carried out about already discovered QTLs before inserting into popular variety through marker assisted selection .
Scientist around the world working to produce waterlogging tolerance varieties of different crops, till date the information about QTLs of waterlogging is very limited and needs to be explored.
The authors would like to acknowledge Long-term Research Grant Scheme (LRGS), Food Security: Enhancing sustainable rice production, Ministry of Higher Education, Malaysia, for the financial support to conduct research activities on rice breeding.
- H. K. Mohanty and G. S. Khush, “Diallel analysis of submergence tolerance in rice, Oryza sativa L,” Theoretical and Applied Genetics, vol. 70, no. 5, pp. 467–473, 1985.
- R. E. Huke and E. H. Huke, Rice Area by Type of Culture South Southeast, and East Asia a Revised and Updated Database, International Rice Research Institute, Los Banos, Philippines, 1997.
- D. Catling, Rice in Deep Water, MacMillan Press, London, UK, 1992.
- D. J. Mackill, W. R. Coffman, and D. P. Garrity, Rainfed Lowland Rice Improvement, International Rice Research Institute, Los Banos, Philippines, 1996.
- V. Buchanan Wollaston, S. Earl, E. Harrison et al., “The molecular analysis of plant senescence—agenomics approach,” Plant Biotechnology Journal, vol. 1, no. 1, pp. 3–22, 2003.
- M. C. Drew and E. J. Sisworo, “Early effects of flooding on nitrogen deficiency and leaf chlorosis in barley,” New Phytologist, vol. 79, no. 3, pp. 567–571, 1977.
- S. Wang, L. He, Z. Li, J. Zeng, Y. Cai, and L. Hou, “A comparative study of the resistance of barley and wheat to waterlogging,” Acta Agronomica Sinica, vol. 22, pp. 228–232, 1996.
- A. I. Malik, T. D. Colmer, H. Lambers, T. L. Setter, and M. Schortemeyer, “Short-term waterlogging has long-term effects on the growth and physiology of wheat,” New Phytologist, vol. 153, no. 2, pp. 225–236, 2002.
- M. C. Drew, “Oxygen deficiency in the root environment and plant mineral nutrition,” in Plant Life Under Oxygen Deprivation, M. B. Jackson, et al., Ed., pp. 301–316, Academic Publishing, The Hague, The Netherlands, 1991.
- Huang Bingru, J. W. Johnson, S. Nesmith, and D. C. Bridges, “Growth, physiological and anatomical responses of two wheat genotypes to waterlogging and nutrient supply,” Journal of Experimental Botany, vol. 45, no. 271, pp. 193–202, 1994.
- T. T. Kozlowski, “Extent, causes, and impact of flooding,” in Flooding and Plant Growth, T. T. Kozlowski, Ed., pp. 9–45, Academic Press, London, UK, 1984.
- T. L. Setter, P. Burgess, I. Water, and J. Kuo, “Genetic diversity of barley and wheat for waterlogging tolerance in Western Australia,” in Proceeding of the 9th Australian Barley Technical Symposium, Melbourne, Australila, 1999.
- T. R. Rathore and M. Z. K. Warsi, “Production of maize under excess soil moisture (waterlogging) conditions,” in Proceedings of the 2nd Asian Regional Maize Workshop PACARD, Laos Banos, Phillipines, February 1998.
- N. Reyna, B. Cornelious, J. G. Shannon, and C. H. Sneller, “Evaluation of a QTL for waterlogging tolerance in Southern Soybean Germplasm,” Crop Science, vol. 43, no. 6, pp. 2077–2082, 2003.
- M. Bacanamwo and L. C. Purcell, “Soybean root morphological and anatomical traits associated with acclimation to flooding,” Crop Science, vol. 39, no. 1, pp. 143–149, 1999.
- G. Linkemer, J. E. Board, and M. E. Musgrave, “Waterlogging effects on growth and yield components in late-planted soybean,” Crop Science, vol. 38, no. 6, pp. 1576–1584, 1998.
- C. J. Daugherty and M. E. Musgrave, “Characterization of populations of rapid-cycling Brassica rapa L. selected for differential waterlogging tolerance,” Journal of Experimental Botany, vol. 45, no. 272, pp. 385–392, 1994.
- T. T. VanToai, J. E. Beuerlein, A. F. Schmitthenner, and S. K. St Martin, “Genetic variability for flooding tolerance in soybeans,” Crop Science, vol. 34, no. 4, pp. 1112–1115, 1994.
- H. D. Scott, J. De Angulo, M. B. Daniels, and L. S. Wood, “Flood duration effects on soybean growth and yield,” Agronomy Journal, vol. 81, no. 2, pp. 631–636, 1989.
- H. D. Scott, J. De Angulo, L. S. Wood, and D. J. Pitts, “Influence of temporary flooding at three growth stages on soybean growth on a clayey soil,” Journal of Plant Nutrition, vol. 13, no. 8, pp. 1045–1071, 1990.
- D. M. Oosterhuis, H. D. Scott, R. E. Hampton, and S. D. Wullschleger, “Physiological responses of two soybean [Glycine max (L.) Merr] cultivars to short-term flooding,” Environmental and Experimental Botany, vol. 30, no. 1, pp. 85–92, 1990.
- C. D. Stanley, T. C. Kaspar, and H. M. Taylor, “Soybean top and root response to temporary water tables imposed at three different stages of growth,” Agronomy Journal, vol. 72, pp. 341–346, 1980.
- J. S. Boyer, “Plant productivity and environment (crop genetic improvement),” Science, vol. 218, no. 4571, pp. 443–448, 1982.
- A. Collaku and S. A. Harrison, “Losses in wheat due to waterlogging,” Crop Science, vol. 42, no. 2, pp. 444–450, 2002.
- M. E. Musgrave and N. Ding, “Evaluating wheat cultivars for waterlogging tolerance,” Crop Science, vol. 38, no. 1, pp. 90–97, 1998.
- H. Alamgir and S. N. Uddin, “Mechanisms of waterlogging tolerance in wheat: morphological and metabolic adaptations under hypoxia or anoxia,” Australian Journal of Crop Science, vol. 5, no. 9, pp. 1094–1110, 2011.
- F. N. Ponnamperuma, “The chemistry of submerged soils,” Advances in Agronomy, vol. 24, pp. 29–96, 1972.
- A. Musgrave, M. B. Jackson, and E. Long, “Gallitriche stem elongation is controlled by ethylene and gibberellin,” Nature New Biology, vol. 238, pp. 93–96, 1972.
- J. T. Stünzi and H. Kende, “Gas composition in the internal air spaces of deepwater rice in relation to growth induced by submergence,” Plant and Cell Physiology, vol. 30, no. 1, pp. 49–56, 1989.
- M. B. Jackson, “Ethylene and responses of plants to soil waterlogging and submergence,” Annual Reviews of Plant Physiology, vol. 36, pp. 145–174, 1985.
- K. J. N. D. Bradford and S. F. Yang, “Xylem transport of 1-aminocyclopropane-1-carboxylic acid, an ethylene precursor, in waterlogged tomato plants,” Plant Physiology, vol. 65, pp. 322–326, 1980.
- E. Cohen and H. Kende, “In vivo 1-aminocyclopropane-1-carboxylate synthase activity in internodes of deep water rice: enhancement by submergence and low oxygen levels,” Plant Physiology, vol. 84, pp. 282–286, 1987.
- Y. Hattori, K. Nagai, S. Furukawa et al., “The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water,” Nature, vol. 460, no. 7258, pp. 1026–1030, 2009.
- H. Kende, E. D. Van Knaap, and H. T. Cho, “Deepwater rice: a model plant to study stem elongation,” Plant Physiology, vol. 118, no. 4, pp. 1105–1110, 1998.
- M. B. Jackson and W. Armstrong, “Formation of aerenchyma and the processes of plant ventilation in relation to soil flooding and submergence,” Plant Biology, vol. 1, no. 3, pp. 274–287, 1999.
- S. Aschi-Smiti, W. Chaïbi, R. Brouquisse, B. Ricard, and P. Saglio, “Assessment of enzyme induction and aerenchyma formation as mechanisms for flooding tolerance in Trifolium subterraneum ‘Park’,” Annals of Botany, vol. 91, pp. 195–204, 2003.
- E. L. J. Watkin, C. J. Thomson, and H. Greenway, “Root development and aerenchyma formation in two wheat cultivars and one Triticale cultivar grown in stagnant agar and aerated nutrient solution,” Annals of Botany, vol. 81, no. 2, pp. 349–354, 1998.
- S. H. F. W. Justin and W. Armstrong, “Evidence for the involvement of ethene in aerenchyma formation in adventitious roots of rice (Oryza sativa),” New Phytologist, vol. 118, pp. 49–62, 2003.
- H. Arikado and Y. Adachi, Anatomical and Ecological Responses of Barley and Some Forage Crops to the Flooding Treatment, vol. 11, Bulletin, Faculty of Agriculture, Mie University, 1955.
- A. H. L. A. N. Gunawardena, D. M. Pearce, M. B. Jackson, C. R. Hawes, and D. E. Evans, “Characterisation of programmed cell death during aerenchyma formation induced by ethylene or hypoxia in roots of maize (Zea mays L.),” Planta, vol. 212, no. 2, pp. 205–214, 2001.
- R. K. Sairam, D. Kumutha, K. Ezhilmathi, P. S. Deshmukh, and G. C. Srivastava, “Physiology and biochemistry of waterlogging tolerance in plants,” Biologia Plantarum, vol. 52, no. 3, pp. 401–412, 2008.
- E. J. W. Visser, G. M. Bögemann, C. W. P. M. Blom, and L. A. C. J. Voesenek, “Ethylene accumulation in waterlogged Rumex plants promotes formation of adventitious roots,” Journal of Experimental Botany, vol. 47, no. 296, pp. 403–410, 1996.
- J. Armstrong and W. Armstrong, “Phragmites: a preliminary study of soil oxidizing sites and internal gas transport pathways,” New Phytologist, vol. 108, no. 4, pp. 373–382, 1988.
- E. J. W. Visser, R. H. M. Nabben, C. W. P. M. Blom, and L. A. C. J. Voesenek, “Elongation by primary lateral roots and adventitious roots during conditions of hypoxia and high ethylene concentrations,” Plant, Cell and Environment, vol. 20, no. 5, pp. 647–653, 1997.
- M. B. Jackson and M. C. Drew, “Effects of flooding on growth and metabolism of herbaceous plants,” in Flooding and Plant Growth, T. T. Kozlowsky, Ed., Academic Press, Orlando, Fla, USA, 1984.
- H. Mergemann and M. Sauter, “Ethylene induces epidermal cell death at the site of adventitious root emergence in rice,” Plant Physiology, vol. 124, no. 2, pp. 609–614, 2000.
- B. B. Vartapetian and M. B. Jackson, “Plant adaptations to anaerobic stress,” Annals of Botany, vol. 79, pp. 3–20, 1997.
- R. M. M. Crawford and R. Braendle, “Oxygen deprivation stress in a changing environment,” Journal of Experimental Botany, vol. 47, no. 295, pp. 145–159, 1996.
- B. Ricard, I. Couee, P. Raymond, P. H. Saglio, V. Saint-Ges, and A. Pradet, “Plant metabolism under hypoxia and anoxia,” Plant Physiology and Biochemistry, vol. 32, no. 1, pp. 1–10, 1994.
- R. A. Kennedy, M. E. Rumpho, and T. C. Fox, “Anaerobic metabolism in plants,” Plant Physiology, vol. 100, no. 1, pp. 1–6, 1992.
- P. Perata and A. Alpi, “Plant responses to anaerobiosis,” Plant Science, vol. 93, no. 1-2, pp. 1–17, 1993.
- A. F. Schmitthenner, “Problems and progress in control of Phytophthora root rot of soybean,” Plant Disease, vol. 69, pp. 362–368, 1985.
- N. R. Fausey, T. T. VanToai, and M. B. McDonald Jr., “Responses of ten common cultivars to flooding,” Transactions of the American Society of Agricultural Engineers, vol. 28, no. 6, pp. 1794–1797, 1985.
- K. A. Barrick and M. G. Noble, “The iron and manganese status of seven upper montane tree species in Colorado, USA, following long-term waterlogging,” Journal of Ecology, vol. 81, no. 3, pp. 523–531, 1993.
- C. J. Thomson, B. J. Atwell, and H. Greenway, “Response of wheat seedlings to low O2 concentration in nutrient solution: II,” Journal of Experimental Botany, vol. 40, pp. 993–999, 1989.
- G. A. F. Hendry and K. J. Brocklebank, “Iron-induced oxygen radical metabolism in waterlogged plants,” New Phytologist, vol. 101, pp. 199–206, 1985.
- D. Hille Ris Lambers and B. S. Vergara, “Summary results of an international collaboration on screening methods for flood tolerance,” in Proceedings of the 1981 International Deepwater Rice Workshop, pp. 347–353, International Rice Research Institute, Los Banos, Philippines, 1982.
- B. S. Vergara and A. Mazaredo, “Screening for resistance to submergence under greenhouse conditions,” in Proceedings of the International Seminar on Deepwater Rice, pp. 67–70, BRRI, Dhaka, Bangladesh, 1975.
- Q. A. Haque, D. Hille Ris Lambers, N. M. Tepora, and Q. D. dela Cruz, “Inheritacne of submergence tolerance in rice,” Euphytica, vol. 41, no. 3, pp. 247–251, 1989.
- H. K. Mohanty and R. C. Chaudhary, “Breeding for submergence tolerance in rice in India,” in Progress in Rainfed Lowland Rice, pp. 191–200, International Rice Research Institute, Manila, Philippines, 1986.
- S. B. Mishra, D. Senadhira, and N. L. Manigbas, “Genetics of submergence tolerance in rice (Oryza sativa L.),” Field Crops Research, vol. 46, no. 1–3, pp. 177–181, 1996.
- D. J. Mackill, M. M. Amante, B. S. Vergara, and S. Sarkarung, “Improved semi dwarf rice lines with tolerance to submergence of seedlings,” Crop Science, vol. 33, pp. 749–775, 1993.
- S. Mallik and S. N. Sen, International Symposium on Rainfed Rice Production Strategy for 21st Century, Assam Agricultural University, Jorhat, India, 1997.
- S. Sarkarung, O. N. Singh, J. K. Roy, A. Vanavichit, and P. Bhekasut, “Breeding strategies for rainfed lowland ecosystem,” in Proceeding of International Rice Research Conference, pp. 709–720, IRRI, Manila, Philippines, 1995.
- C. W. Stuber, “Enhancement of grain yield in maize hybrids using marker-facilitated introgression of QTLs in analysis of molecular marker data,” in Proceedings of the Joint American Society of Horticulture Science/Crop Science Society of America Plant Breeding Symposium, Corvalis, 1994.
- M. S. Davies and G. C. Hillman, “Effects of soil flooding on growth and grain yield of populations of tetraploid and hexaploid species of wheat,” Annals of Botany, vol. 62, no. 6, pp. 597–604, 1988.
- G. Boru, M. Van Ginkel, W. E. Kronstad, and L. Boersma, “Expression and inheritance of tolerance to waterlogging stress in wheat,” Euphytica, vol. 117, no. 2, pp. 91–98, 2001.
- A. Collaku and S. A. Harrison, “Heritability of waterlogging tolerance in wheat,” Crop Science, vol. 45, no. 2, pp. 722–727, 2005.
- K. Xu, X. Xu, T. Fukao et al., “Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice,” Nature, vol. 442, no. 7103, pp. 705–708, 2006.
- H. Li, R. Vaillancourt, N. Mendham, and M. Zhou, “Comparative mapping of quantitative trait loci associated with waterlogging tolerance in barley (Hordeum vulgare L.),” BMC Genomics, vol. 9, article 401, 2008.
- F. Qiu, Y. Zheng, Z. Zhang, and S. Xu, “Mapping of QTL associated with waterlogging tolerance during the seedling stage in maize,” Annals of Botany, vol. 99, no. 6, pp. 1067–1081, 2007.
- T. T. Vantoai, S. K. S. Martin, K. Chase et al., “Identification of a QTL associated with tolerance of soybean to soil waterlogging,” Crop Science, vol. 41, no. 4, pp. 1247–1252, 2001.
- B. Cornelious, P. Chen, Y. Chen, N. De Leon, J. G. Shannon, and D. Wang, “Identification of QTLs underlying water-logging tolerance in soybean,” Molecular Breeding, vol. 16, no. 2, pp. 103–112, 2005.
- H. K. Mohanty, B. Suprihatno, G. S. Khush, W. R. Coffman, and B. S. Vergara, “Inheritance of submergence tolerance in deepwater rice,” in Proceedings of the International Deepwater Rice Workshop, pp. 121–134, International Rice Research Institute, Los Banos, Philippines, 1982.
- B. Suprihatno and W. R. Coffman, “Inheritance of submergence tolerance in rice (Oryza sativa L),” SABRAO Journal, vol. 13, pp. 98–102, 1981.
- K. Xu and D. J. Mackill, “A major locus for submergence tolerance mapped on rice chromosome 9,” Molecular Breeding, vol. 2, no. 3, pp. 219–224, 1996.
- S. Nandi, P. K. Subudhi, D. Senadhira, N. L. Manigbas, S. Sen-Mandi, and N. Huang, “Mapping QTLs for submergence tolerance in rice by AFLP analysis and selective genotyping,” Molecular and General Genetics, vol. 255, no. 1, pp. 1–8, 1997.
- M. Siangliw, T. Toojinda, S. Tragoonrung, and A. Vanavichit, “Thai jasmine rice carrying QTLch9 (SubQTL) is submergence tolerant,” Annals of Botany, vol. 91, pp. 255–261, 2003.
- T. Toojinda, M. Siangliw, S. Tragoonrung, and A. Vanavichit, “Molecular genetics of submergence tolerance in rice: QTL analysis of key traits,” Annals of Botany, vol. 91, pp. 243–253, 2003.
- K. Xu, X. Xu, P. C. Ronald, and D. J. Mackill, “A high-resolution linkage map of the vicinity of the rice submergence tolerance locus Sub1,” Molecular and General Genetics, vol. 263, no. 4, pp. 681–689, 2000.
- T. Fukao, T. Harris, and J. Bailey-Serres, “Evolutionary analysis of the Sub1 gene cluster that confers submergence tolerance to domesticated rice,” Annals of Botany, vol. 103, no. 2, pp. 143–150, 2009.
- T. Fukao, K. Xu, P. C. Ronald, and J. Bailey-Serres, “A variable cluster of ethylene response factor-like genes regulates metabolic and developmental acclimation responses to submergence in rice,” Plant Cell, vol. 18, no. 8, pp. 2021–2034, 2006.
- S. Singh, D. J. Mackill, and A. M. Ismail, “Responses of SUB1 rice introgression lines to submergence in the field: yield and grain quality,” Field Crops Research, vol. 113, no. 1, pp. 12–23, 2009.
- Z. Zhang, H. Jiang, Z. Wei, and Y. Zheng, “Study on enzymology in root of maize inbred after waterlogging stress,” Hubei Agricultural Sciences, vol. 3, pp. 25–27, 2003.
- W. Tang, Z. Zhang, X. Zou, and Y. Zheng, “Functional genomics of maize submergence tolerance and cloning of the related gene Sicyp51,” Science in China, Series C, vol. 48, no. 4, pp. 337–345, 2005.
- D. J. Mackill, “Breeding for resistance to abiotic stresses in rice: the value of quantitative trait loci,” in Plant Breeding: The Arnel, R Hallauer International Symposium Ames, K. R. Lamkey and M. Lee, Eds., pp. 201–212, Blackwell, 2006.
- K. Xu, R. Deb, and D. J. Mackill, “A microsatellite marker and a co-dominant PCR based marker for marker assisted selection of submergence tolerance in rice,” Crop Science, vol. 44, no. 1, pp. 248–253, 2004.
- E. M. Septiningsih, A. M. Pamplona, D. L. Sanchez et al., “Development of submergence-tolerant rice cultivars: the Sub1 locus and beyond,” Annals of Botany, vol. 103, no. 2, pp. 151–160, 2009.
- C. N. Neeraja, R. Maghirang-Rodriguez, A. Pamplona et al., “A marker-assisted backcross approach for developing submergence-tolerant rice cultivars,” Theoretical and Applied Genetics, vol. 115, no. 6, pp. 767–776, 2007.
- R. K. Sarkar, D. Panda, J. N. Reddy, S. S. C. Patnaik, D. J. Mackill, and A. M. Ismail, “Performance of submergence tolerant rice (Oryza sativa) genotypes carrying the Sub1 quantitative trait locus under stressed and non-stressed natural field conditions,” Indian Journal of Agricultural Sciences, vol. 79, no. 11, pp. 876–883, 2009.
- R. K. Sarkar, J. N. Reddy, S. G. Sharma, and A. M. Ismail, “Physiological basis of submergence tolerance in rice and implications for crop improvement,” Current Science, vol. 91, no. 7, pp. 899–906, 2006.
- D. O. Manzanilla, T. R. Paris, G. V. Vergara et al., “Submergence risks and farmers' preferences: implications for breeding Sub1 rice in Southeast Asia,” Agricultural Systems, vol. 104, no. 4, pp. 335–347, 2011.
- A. Mukhopadhyay, D. Minhas, and A. Grover, “Callusing from rice root explants: adventitious root formation precedes callus initiation response,” Current Science, vol. 73, no. 5, pp. 465–469, 1997.
- D. Minhas and A. Grover A, “Towards developing transgenic rice plants tolerant to flooding stress,” in Proceedings of the Indian National Science Academy, vol. B65, pp. 33–50, 1999.
- A. Grover, M. A. Hossain, M. E. Huq et al., International Rice Research Conference, IRRI, Manila, Philippines, 1995.
- A. Grover, M. Rahman, M. Ellis et al., General Meeting of the International Programme on Rice Biotechnology, Rockefeller Foundation, Malacca, Malaysia, 1997.
- A. Grover, M. Rahman, D. Minhas et al., General Meeting of the International Programme on Rice Biotechnology, Rockefeller Foundation, Phuket, Thailand, 1999.
- C. A. Quimio, L. B. Torrizo, T. L. Setter et al., “Enhancement of submergence tolerance in transgenic rice overproducing pyruvate decarboxylase,” Journal of Plant Physiology, vol. 156, no. 4, pp. 516–521, 2000.
- E. S. Dennis, R. Dolferus, M. Ellis et al., “Molecular strategies for improving waterlogging tolerance in plants,” Journal of Experimental Botany, vol. 51, no. 342, pp. 89–97, 2000.
- P. Wenzl, J. Carling, D. Kudrna et al., “Diversity Arrays Technology (DArT) for whole-genome profiling of barley,” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 26, pp. 9915–9920, 2004.
- S. A. Goff, D. Ricke, T. H. Lan et al., “A draft sequence of the rice genome (Oryza sativa L. ssp. japonica),” Science, vol. 296, no. 5565, pp. 92–100, 2002.
- T. Sasaki, “The map-based sequence of the rice genome,” Nature, vol. 436, no. 7052, pp. 793–800, 2005.
- N. Yamada, “Physiological basis of resistance of rice plant against overhead flooding,” Bulletin of the National Institute of Agricultural Sciences, Series D, vol. 8, pp. 1–112, 1959.
- B. Rathinasabapathi, “Metabolic engineering for stress tolerance: installing osmoprotectant synthesis pathways,” Annals of Botany, vol. 86, no. 4, pp. 709–716, 2000.
- Y. Mano, M. Muraki, and T. Takamizo, “Identification of QTL controlling flooding tolerance in reducing soil conditions in maize (Zea mays L.) seedlings,” Plant Production Science, vol. 9, no. 2, pp. 176–181, 2006.