An Insight into the “-Omics” Based Engineering of Streptomycetes for Secondary Metabolite Overproduction

Table 3

Methodologies used to overproduce drugs using engineered genomics approach.

Strains	Drugs	Approach	Methodologies
S. kanamyceticus	Kanamycin	Genome guided overexpression of gene clusters in native and heterologous hosts	Overexpression of extra copy of the gene cluster enhanced kanamycin production
S. ansochromogenes	Nikkomycin	Genome guided overexpression of gene clusters in native and heterologous hosts	Overexpression of extra copy of the gene cluster enhanced nikkomycins production
S. coelicolor	Actinorhodin	Genome guided overexpression of gene clusters in native and heterologous hosts	Tandem copies of the gene cluster increased actinorhodin production
S. avermitilis	Streptomycin, cephamycin C, and pladienolide	Genome guided overexpression of gene clusters in native and heterologous hosts	Heterologous expression in genome-minimized strain
S. venezuelae YJ003	Tylosin, kanamycin, spectinomycin, spectinamine, gentamicin, and epothilones	Genome guided overexpression of gene clusters in native and heterologous hosts	Heterologous expression in pikromycin gene cluster deleted strain
S. lividans TK-23, TK-24, and TK-63	Daptomycin and paromamine	Genome guided overexpression of gene clusters in native and heterologous hosts	Heterologous expression
S. lividans	Capreomycin	Genome guided overexpression of gene clusters in native and heterologous hosts	Heterologous expression
S. albus J1074	Thiocoraline	Genome guided overexpression of gene clusters in native and heterologous hosts	Heterologous expression
S. fradiae	Tylosin	Genome shuffling guided enhancement of secondary metabolites	Two rounds of genome shuffling
S. gilvosporeus SG1	Natamycin	Genome shuffling guided enhancement of secondary metabolites	Four rounds of genome shuffling
S. pristinaespiralis	Pristinamycin	Genome shuffling guided enhancement of secondary metabolites	Four rounds of genome shuffling to increase the resistivity against pristinamycin enhanced pristinamycin production
S. sp. U121	(2S, 3R)-HCA	Genome shuffling guided enhancement of secondary metabolites	Generating resistance mechanism for transepoxyaconitic acid by three rounds of shuffling
S. padanus,; S. griseofuscus,; S. graminearus,; S. hygroscopicus, and S. albulus	ε-Poly-l-lysine	Genome shuffling guided enhancement of secondary metabolites	Through glucose, sulfa guanidine, and succinic acid tolerance and genome shuffling