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Strains | Drugs | Approach | Methodologies |
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S. venezuelae | Pikromycin | Proteomics facilitated reverse engineering to enhance secondary metabolite production | Overexpression of the metK gene encoding SAM synthetase |
S. griseus IFO13189 and S. griseoflavus FERM1805 | Spectinomycin and bicozamycin | Proteomics facilitated reverse engineering to enhance secondary metabolite production | Exogenous feeding of S-Adenosyl methionine (SAM) results in enhanced production |
S. sp. FR-008, S. avermitilis, S. coelicolor A3 (2), S. lividans TK23, and S. antibioticus ATCC11891 | Candicin D, avermectin, actinorhodin, and oleandomycin | Proteomics facilitated reverse engineering to enhance secondary metabolite production | Coexpression of metK or exogenous feeding of SAM enhanced antibiotic production |
S. avermitilis | Avermectins | Proteomics facilitated reverse engineering to enhance secondary metabolite production | Overexpression of mutant library of sigma factor enhanced antibiotic production |
S. peucetius ATCC 27952 | Doxorubicin | Proteomics facilitated reverse engineering to enhance secondary metabolite production | Overexpression of efflux protein DrrA enhanced antibiotic production |
S. lividans TK24 | Actinorhodin | Ribosome engineering to enhance secondary metabolite production | Resistance against streptomycin causes production of pigmented antibiotic actinorhodin not produced in normal laboratory conditions |
S. chattanoogensis | Fredericamycin | Ribosome engineering to enhance secondary metabolite production | Resistance against streptomycin causes enhanced production |
S. lividans 66 | Actinorhodin, undecylprodigiosin, and calcium dependent antibiotics | Ribosome engineering to enhance secondary metabolite production | By introducing rifampicin mutations into the rpoB (encoding the RNA polymerase subunit) gene |
S. coelicolor A3 (2) | Actinorhodin | Ribosome engineering to enhance secondary metabolite production | By introducing double and triple mutations using gentamicin rifampicin and streptomycin increases actinorhodin production |
S. coelicolor A3 (2) | Actinorhodin | Ribosome engineering to enhance secondary metabolite production | Enhanced expression of ribosome recycling factor by mutation increases production |
S. avermitilis | Avermectin | Ribosome engineering to enhance secondary metabolite production | Overexpression of ribosome recycling factor increases production |
S. coelicolor A3 (2) | Chloramphenicol and congocidine | Ribosome engineering to enhance secondary metabolite production | Introducing rpsL and rpoB mutations enhanced chloramphenicol and congocidine production |
Streptomycetes | Antibiotics | Ribosome engineering to enhance secondary metabolite production | Introducing mutations in rsmG gene encoding for 16S rRNA methyltransferase |
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