| Table 2: Box-Behnken design for the Ca(OH)2 pretreatment of rice straw. |
| | Run | Coded | Uncoded | Response | LC (%) | PT (d) | IA (%) | LC (%) | PT (d) | IA (%) | Methane yield (mL/g VS) |
| | 1 | 0 | −1 | −1 | 8 | 3 | 20 | 154.0 | | 2 | 0 | 1 | 1 | 8 | 11 | 50 | 200.0 | | 3 | 0 | 0 | 0 | 8 | 7 | 35 | 200.0 | | 4 | 1 | −1 | 0 | 12 | 3 | 35 | 156.5 | | 5 | 0 | 0 | 0 | 8 | 7 | 35 | 215.1 | | 6 | 1 | 0 | 1 | 12 | 7 | 50 | 190.0 | | 7 | 0 | 1 | −1 | 8 | 11 | 20 | 155.9 | | 8 | 0 | 0 | 0 | 8 | 7 | 35 | 207.1 | | 9 | 1 | 0 | −1 | 12 | 7 | 20 | 103.6 | | 10 | 0 | 0 | 0 | 8 | 7 | 35 | 228.2 | | 11 | 0 | 0 | 0 | 8 | 7 | 35 | 229.0 | | 12 | −1 | 0 | −1 | 4 | 7 | 20 | 167.0 | | 13 | −1 | 0 | 1 | 4 | 7 | 50 | 190.0 | | 14 | −1 | 1 | 0 | 4 | 11 | 35 | 167.0 | | 15 | 1 | 1 | 0 | 12 | 11 | 35 | 141.1 | | 16 | −1 | −1 | 0 | 4 | 3 | 35 | 190.0 | | 17 | 0 | −1 | 1 | 8 | 3 | 50 | 216.1 |
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LC: Ca(OH)2 concentration; PT: pretreatment time; IA: inoculum amount.
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