Research Article
The Mode of Inheritance of Scheuermann’s Disease
Table 1
Primers and conditions for cartilage-related mesoderm marker RT-PCR.
| Gene name | Primers name | Primer sequence | MgCl2 concentration, mM | Annealing temperature, °C | Target fragment size, bp |
| AGGRE | ACAN f ACAN r | 5′-ATCGTCACCCCCGAGGAGCAG-3′ 5′-GGCGCTGGACAAACCCCTCTG-3′ | 3 | 60 | 379 |
| LUM | LUM2 f LUM2 r | 5′-GTGACTGGGCTGGGTCTCCCC-3′ 5′-GGCACTTGGGTAGCTTTCAGGGC-3′ | 4 | 58 | 329 |
| HAPLN1 | HAPLN1 f HAPLN1 r | 5′-GGCAGAACACAGTGCCCGGAGTC-3′ 5′-GCGCACTGCAGCCTCAGTAGGAC-3′ | 4 | 58 | 317 |
| GHR | GHR f GHR r | 5′-GGCGAAGCTCGGAGGTCCTACA-3′ 5′-TGCATTGCGTGGTGCTTCCCATC-3′ | 4 | 58 | 568 |
| TGFBR | TGFBR f TGFBR r | 5′-GGCGAGCGGTCTTGCCCATC-3′ 5′-CTCAAGGCTTCACAGCTCTGCCA-3′ | 4 | 58 | 468 |
| COL1A1 | COL1A1 f COL1A1 r | 5′-ATCCAGCTGACCTTCCTGCG-3′ 5′-TGGAAGCCGAATTCCTGGTCT-3′ | 4 | 58 | 301 |
| COL2A1 | COL2A1 f COL2A1 r | 5′-GAAACCATCAATGGTGGCTTCC-3′ 5′-CGATAACAGTCTTGCCCCACTT-3′ | 4 | 58 | 322 |
| IHH3 | IHH3 f IHH3 r | 5′-ACCACATCAGACCGCGACCGCAAT-3′ 5′-AGGCTGCACGTGGCTGGCAAATGT-3′ | 4 | 58 | 441 |
| SOX9 | SOX9 f SOX9 r | 5′-GCGTCAACGGCTCCAGCAAGA-3′ 5′-GGCCTGCAGCGCCTTGAAGAT-3′ | 4 | 58 | 369 |
| PAX1 | PAX1 f PAX1 r | 5′-ATCAGCCGCATCCTGCGCAACAA-3′ 5′-TCTCTAGCCCATTCACTGCGGGGT-3′ | 4 | 65 | 376 |
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