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Figure 5: Transduction of cerebellar Purkinje cells. Immunohistochemical detection of GFP and calbindin, a Purkinje cell marker, in mouse cerebellums following intracardiac injections of tyrosine-mutant AAV9/3 vectors that expressed GFP under control of the L7 promoter. (a) An axial section demonstrating colabeling of GFP (green) and calbindin (red) in the cerebellar cortex. ((b)–(d)) High magnification images of the rectangular area outlined in (a). (b) GFP-positive cells (green); (c) Calbindin-positive cells (red); and (d) the merged image of ((b) and (c)). Scale bars: (a) 100 μm; ((b)–(d)) 30 μm.