Research Article
Single Nucleotide Polymorphism in the Promoter of the Human Interleukin-13 Gene Is Associated with Asthma in Malaysian Adults
Table 1
Technical data for the analysis of single nucleotide polymorphisms (SNPs) in the human IL-13 gene.
| Region | Primers | PCR product size (bp) | Restriction enzyme* | Annealing temperature | Reference |
| Promoter: (−1111C>T) | Forward: Reverse: | 5′-ACT TCT GGG AGT CAG AGC CA-3′ 5′-TAC AGC CAT GTC GCC TTT TCC TGC TCT TCC GTC-3′ | 372 | Hpy99I | 60°C | [22] |
| Coding: (4257G>A) | Forward: Reverse: | 5′-CTT CCG TGA GGA CTG AAT GAG ACG GTC-3′ 5′-GCA AAT AAT GAT GCT TTC GAA GTT TCA GTG GA-3′ | 236 | NlaIV | 60°C | [23] |
|
|
GenBank accession number U31120 was used as the reference sequence.
*New England Biolabs, Beverly, MA, USA.
|