Table 1: Technical data for the analysis of single nucleotide polymorphisms (SNPs) in the human IL-13 gene.

RegionPrimersPCR product size (bp)Restriction enzyme*Annealing temperature Reference

Promoter:
(−1111C>T)
Forward:
Reverse:
5′-ACT TCT GGG AGT CAG AGC CA-3′  
5′-TAC AGC CAT GTC GCC TTT TCC TGC TCT TCC GTC-3′
372Hpy99I 60°C[22]

Coding:
(4257G>A)
Forward:
Reverse:
5′-CTT CCG TGA GGA CTG AAT GAG ACG GTC-3′  
5′-GCA AAT AAT GAT GCT TTC GAA GTT TCA GTG GA-3′
236NlaIV60°C[23]

GenBank accession number U31120 was used as the reference sequence.
*New England Biolabs, Beverly, MA, USA.