Exercise Training and Work Task Induced Metabolic and Stress-Related mRNA and Protein Responses in Myalgic Muscles

Table 1

Primer and TaqMan probe sequences for real-time PCR.

Gene	Forward primer	Reverse primer	Taqman probe
GLUT4	5′ CCTGCCAGAAAGAGTCTGAAGC 3′	5′ ATCCTTCAGCTCAGCCAGCA 3′	5′ CAGAAACATCGGCCCAGCCTGTCA 3′
GS	5′ GCTCAGAGCAAGGCTCGAAT 3′	5′ CGGCCGGCGATAAAGAA 3′	5′ TTATGGGCATCTGGACTTCAACTTGGACA 3′
HIF-1α	5′ GCCCCAGATTCAGGATCAGA 3′	5′ TGGGACTATTAGGCTCAGGTGAAC 3′	5′ ACCTAGTCCTTCCGATGGAAGCACTAGACAA 3′
HO-1	5′ GCCAGCAACAAAGTGCAAGAT 3′	5′ AGTGTAAGGACCCATCGGAGAA 3′	5′ AGAGGGAAGCCCCCACTCAACACC 3′
Hsc70	5′ GCAGACACAGACCTTCACTACCTATT 3′	5′ GGCACGCTCGCCTTCAT 3′	5′ AACCTGAATAAGCACACCAGGCTGGTTG 3′
HSP72	5′ GCGTGATGACTGCCCTGAT 3′	5′ CGCCCTCGTACACCTGGAT 3′	5′ TCCCCACCAAGCAGACGCAGATCT 3′
IGF-IEa	5′ CAGCGCCACACCGACAT 3′	5′ TTGTTTCCTGCACTCCCTCTACT 3′	5′ AAGACCCAGAAGGAAGTACATTTGAAGAACGC 3′
IL-6	5′ TCTCAGCCCTGAGAAAGGAGACA 3′	5′ CATCTTTGGAAGGTTCAGGTTGT 3′	5′ ACATGTGTGAAAGCAGCAAAGAGGCACTG 3′
LDH-A	5′ ACAACAGGATTCTAGGTGGAGGTT 3′	5′ GAGTTGATGTTTTTCCCAGTCCAT 3′	5′ TGCATGTTGTCCTTTTTATCTGATCTGTGATTAAAGC 3′
LDH-B	5′ GCTAAAGGATGATGAGGTTGCTC 3′	5′ TCACTAGTCACAGGTCTTTTAGGTCC 3′	5′ CTCAAGAAAAGTGCAGATACCCTGTGGGAC 3′
Myostatin	5′ ACCAGGAGAAGATGGGCTGAA 3′	5′ GTCAAGACCAAAATCCCTTCTGGA 3′	5′ CCGTTTTTAGAGGTCAAGGTAACAGACACACCA 3′
PGC-1α	5′ CAAGCCAAACCAACAACTTTATCTCT 3′	5′ CACACTTAAGGTGCGTTCAATAGTC 3′	5′ AGTCACCAAATGACCCCAAGGGTTCC 3′
TNFα	5′ TCTGGCCCAGGCAGTCAGAT 3′	5′ AGCTGCCCCTCAGCTTGA 3′	5′ CAAGCCTGTAGCCCATGTTGTAGCAAACC 3′

GS: glycogen synthase; HIF-1α: hypoxia inducible factor-1; HO-1: heme oxygenase-1; HSP72 and HSc70: heat shock protein 70 and 72; IGF-1Ea: insulin-like growth factor-1Ea; IL-6: interleukin-6; LDH: lactate dehydrogenase; PGC-1α: peroxisome proliferator activated receptor gamma coactivator 1α; TNF-α: tumor necrosis factor alpha.