Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus
Figure 3
mRNA expression of defensin PaDef of avocado in BVE-E6E7 endothelial cells. (a) RT-PCR analysis that shows the amplification of defensin PaDef in polyclonal population of BVE-E6E7 cells transfected (BVE-PC). The lack of defensin PaDef amplification is shown in nontransfected BVE-E6E7 cells (BVE-E6E7) or only transfected with the vector (BVpT). Also, the positive control is included (pBME3). The 1 kb molecular weight marker (Invitrogen) was also included (MW). (b) RT-PCR analysis that shows the amplification of defensin PaDef in different endothelial cell clones.
