About this Journal Submit a Manuscript Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 986273, 9 pages
http://dx.doi.org/10.1155/2013/986273
Research Article

Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus

1Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Km 9.5 Carretera Morelia-Zinapécuaro, Posta Veterinaria, 58893 Morelia, MICH, Mexico
2Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo (UMSNH), Ciudad Universitaria, Edificio B1, 58030 Morelia, MICH, Mexico
3Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, A.C. Calle 43 No. 130 Col. Chuburná de Hidalgo, 97200 Mérida, YUC, Mexico

Received 2 November 2012; Accepted 4 September 2013

Academic Editor: Lalji Singh

Copyright © 2013 Jaquelina Julia Guzmán-Rodríguez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. O. E. Heuer, A. M. Hammerum, P. Collignon, and H. C. Wegener, “Human health hazard from antimicrobial-resistant enterococci in animals and food,” Clinical Infectious Diseases, vol. 43, no. 7, pp. 911–916, 2006. View at Publisher · View at Google Scholar · View at Scopus
  2. D. Field, L. Quigley, P. M. O'Connor et al., “Studies with bioengineered Nisin peptides highlight the broad-spectrum potency of Nisin V,” Microbial Biotechnology, vol. 3, no. 4, pp. 473–486, 2010. View at Publisher · View at Google Scholar · View at Scopus
  3. K. Papadopoulou, R. E. Melton, M. Leggett, M. J. Daniels, and A. E. Osbourn, “Compromised disease resistance in saponin-deficient plants,” Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 22, pp. 12923–12928, 1999. View at Publisher · View at Google Scholar · View at Scopus
  4. I. Néeman, A. Lifshitz, and Y. Kashman, “New antibacterial agent isolated from the avocado pear,” Applied microbiology, vol. 19, no. 3, pp. 470–473, 1970. View at Scopus
  5. D. Prusky, N. T. Keen, J. J. Sims, and S. L. Midland, “Possible involvement of an antifungal diene in the latency of Colletotrichum gloeosporioides on unripe avocado fruits,” Phytopathology, vol. 72, no. 12, pp. 1578–1582, 1982. View at Publisher · View at Google Scholar
  6. D. Prusky and N. T. Keen, “Involvement of preformed antifungal compounds in the resistance of subtropical fruits to fungal decay,” Plant Disease, vol. 77, no. 2, pp. 114–119, 1993.
  7. F. Domergue, G. L. Helms, D. Prusky, and J. Browse, “Antifungal compounds from idioblast cells isolated from avocado fruits,” Phytochemistry, vol. 54, no. 2, pp. 183–189, 2000. View at Publisher · View at Google Scholar · View at Scopus
  8. M. D. Wigg, A. A. Al-Jabri, S. S. Costa, E. Race, B. Bodo, and U. S. Oxford, “In vitro virucidal and virustatic anti HIV-1 effects of extracts from Persea americana Mill. (avocado) leaves,” Antiviral Chemistry and Chemotherapy, vol. 7, no. 4, pp. 179–183, 1996. View at Scopus
  9. A. P. de Almeida, M. M. Miranda, I. C. Simoni, M. D. Wigg, M. H. C. Lagrota, and S. S. Costa, “Flavonol monoglycosides isolated from the antiviral fractions of Persea americana (Lauraceae) leaf infusión,” Phytotherapy Research, vol. 12, no. 8, pp. 562–567, 1998.
  10. J. D. L. Sánchez-Pérez, M. G. Jaimes-Lara, R. Salgado-Garciglia, and J. E. López-Meza, “Root extracts from Mexican avocado (Persea americana var. drymifolia) inhibit the mycelial growth of the oomycete Phytophthora cinnamomi,” European Journal of Plant Pathology, vol. 124, no. 4, pp. 595–601, 2009. View at Publisher · View at Google Scholar · View at Scopus
  11. J. E. López-Meza, A. Ochoa-Zarzosa, J. A. Aguilar, and P. D. Loeza-Lara, “Antimicrobial peptides: diversity and perspectives for their biomedical application, biomedical engineering, trends, research and technologies,” in Biomedical Engineering, Trends, Research and Technologies, M. A. Komorowska and S. Olsztynska-Janus, Eds., pp. 275–304, Intech, Rijeka, Croatia, 2011.
  12. B. P. H. J. Thomma, B. P. A. Cammue, and K. Thevissen, “Plant defensins,” Planta, vol. 216, no. 2, pp. 193–202, 2002. View at Publisher · View at Google Scholar · View at Scopus
  13. F. T. Lay and M. A. Anderson, “Defensins—components of the innate immune system in plants,” Current Protein and Peptide Science, vol. 6, no. 1, pp. 85–101, 2005. View at Publisher · View at Google Scholar · View at Scopus
  14. F. García-Olmedo, P. Rodríguez, A. Molina, et al., “Antibiotic activities of peptides, hydrogen peroxide and peroxynitrite in plant defence,” FEBS Letters, vol. 498, no. 2-3, pp. 219–222, 2001.
  15. J. L. Anaya-López, J. E. López-Meza, V. M. Baizabal-Aguirre, H. Cano-Camacho, and A. Ochoa-Zarzosa, “Fungicidal and cytotoxic activity of a Capsicum chinense defensin expressed by endothelial cells,” Biotechnology Letters, vol. 28, no. 14, pp. 1101–1108, 2006. View at Publisher · View at Google Scholar · View at Scopus
  16. H. Loeza-Ángeles, E. Sagrero-Cisneros, L. Lara-Zárate, E. Villagómez-Gómez, J. E. López-Meza, and A. Ochoa-Zarzosa, “Thionin Thi2.1 from Arabidopsis thaliana expressed in endothelial cells shows antibacterial, antifungal and cytotoxic activity,” Biotechnology Letters, vol. 30, no. 10, pp. 1713–1719, 2008. View at Publisher · View at Google Scholar · View at Scopus
  17. M. Cajero-Juárez, B. Avila, A. Ochoa et al., “Immortalization of bovine umbilical vein endothelial cells: amodel for the study of vascular endothelium,” European Journal of Cell Biology, vol. 81, no. 1, pp. 1–8, 2002. View at Scopus
  18. R. López-Gómez and M. A. Gómez-Lim, “A method for extracting intact RNA from fruits rich in polysaccharides using ripe mango mesocarp,” HortScience, vol. 27, no. 5, pp. 440–442, 1992.
  19. B. Ewing and P. Green, “Base-calling of automated sequencer traces using phred. II. Error probabilities,” Genome Research, vol. 8, no. 3, pp. 186–194, 1998. View at Scopus
  20. S. Li and H.-H. Chou, “Lucy2: an interactive DNA sequence quality trimming and vector removal tool,” Bioinformatics, vol. 20, no. 16, pp. 2865–2866, 2004. View at Publisher · View at Google Scholar · View at Scopus
  21. R. López-Gómez, L. E. Ibarra, R. L. M. Suárez, et al., “First insights into the avocado fruit transcriptome,” in Proceedings of the 28th International Horticultural Science Congress, Lisboa, Portugal, August 2010.
  22. M. A. Larkin, G. Blackshields, N. P. Brown et al., “Clustal W and Clustal X version 2.0,” Bioinformatics, vol. 23, no. 21, pp. 2947–2948, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. A. Ochoa-Zarzosa, H. Loeza-Ángeles, E. Sagrero-Cisneros, E. Villagómez-Gómez, L. Lara-Zárate, and J. E. López-Meza, “Antibacterial activity of thionin Thi2.1 from Arabidopsis thaliana expressed by bovine endothelial cells against Staphylococcus aureus isolates from bovine mastitis,” Veterinary Microbiology, vol. 127, no. 3-4, pp. 425–430, 2008. View at Publisher · View at Google Scholar · View at Scopus
  24. N. Alva-Murillo, A. Ochoa-Zarzosa, and J. E. López-Meza, “Short chain fatty acids (propionic and hexanoic) decrease Staphylococcus aureus internalization into bovine mammary epithelial cells and modulate antimicrobial peptide expression,” Veterinary Microbiology, vol. 155, no. 2–4, pp. 324–331, 2012. View at Publisher · View at Google Scholar · View at Scopus
  25. B. Jahn, E. Martin, A. Stueben, and S. Bhakdi, “Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide assay,” Journal of Clinical Microbiology, vol. 33, no. 3, pp. 661–667, 1995. View at Scopus
  26. M. Yasir, S. Das, and M. Kharya, “The phytochemical and pharmacological profile of Persea americana Mill,” Pharmacognosy Reviews, vol. 4, no. 7, pp. 77–84, 2010. View at Publisher · View at Google Scholar · View at Scopus
  27. J. R. King and R. J. Knight, “Occurrence and assay of estragole in the leaves of various avocado cultivars,” Journal of Agricultural and Food Chemistry, vol. 35, no. 5, pp. 842–844, 1987. View at Scopus
  28. C. Rodríguez-Saona and J. T. Trumble, “Secretory avocado idioblast oil cells: evidence of their defensive role against a non-adapted insect herbivore,” Entomologia Experimentalis et Applicata, vol. 94, no. 2, pp. 183–194, 2000. View at Publisher · View at Google Scholar · View at Scopus
  29. L. Padovan, M. Scocchi, and A. Tossi, “Structural aspects of plant antimicrobial peptides,” Current Protein and Peptide Science, vol. 11, no. 3, pp. 210–219, 2010. View at Publisher · View at Google Scholar · View at Scopus
  30. U. S. Sagaram, R. Pandurangi, J. Kaur, T. J. Smith, and D. M. Shah, “Structure-activity determinants in antifungal plant defensins msdef1 and mtdef4 with different modes of action against Fusarium graminearum,” PLoS ONE, vol. 6, no. 4, Article ID e18550, 2011. View at Publisher · View at Google Scholar · View at Scopus
  31. A. Segura, M. Moreno, A. Molina, and F. García-Olmedo, “Novel defensin subfamily from spinach (Spinacia oleracea),” FEBS Letters, vol. 435, no. 2-3, pp. 159–162, 1998. View at Publisher · View at Google Scholar · View at Scopus