Protective Effects of Testosterone on Presynaptic Terminals against Oligomeric -Amyloid Peptide in Primary Culture of Hippocampal Neurons

Figure 4

Testosterone attenuated oligomeric Aβ-induced reduction of synapsin-1 in primary hippocampal neurons. Primary hippocampal neurons were treated with 10 nM testosterone for 1 h, followed by exposure to 5 μM oligomeric Aβ for 24 h. Neurons were stained with synapsin-1 antibody. (a) Control, (b) 10 nM testosterone for 25 h, (c) 5 μM Aβ for 24 h, and (d) 10 nM testosterone for 1 h, followed by exposure to 5 μM Aβ for 24 h. (e) The number of puncta and (f) the fluorescent intensity were analyzed by Image J software as described in the above section. ^∗∗∗𝑃<0.0005 versus control group, ^#𝑃<0.05 versus control group, and ^∗𝑃<0.05 versus Aβ group. White dot boxes indicate reduced number of puncta along neurites.

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