Protective Effects of Testosterone on Presynaptic Terminals against Oligomeric -Amyloid Peptide in Primary Culture of Hippocampal Neurons
Figure 6
Testosterone attenuated oligomeric Aβ-induced reduction of heat shock protein in primary hippocampal neurons. Primary hippocampal neurons were treated with 10 nM testosterone for 1 h, followed by exposure to 5 μM oligomeric Aβ for 24 h. Neurons were stained with HSP70 antibody. (a) Control, (b) 10 nM testosterone for 25 h, (c) 5 μM Aβ for 24 h, and (d) 10 nM testosterone for 1 h, followed by exposure to 5 μM Aβ for 24 h. (e) The fluorescent intensity was analyzed by Image J software as described in the above section. versus control group; versus Aβ group.