Production of Biodiesel from Chlorella sp. Enriched with Oyster Shell Extracts

Table 3

Results of estimating lipid productivity and the fatty acid profiles of cells grown from several culture media under different culture conditions.

			Culture time
Parameter			21 days (6 days of N depletion)			27 days; (12 days of N depletion)
			A	B	C	A	B	C
	Lipid productivity (mg/L/day)		25.5	19.2	20.6	28.8	23.4	25.3
Culture; medium	Total lipids; (%, w/w)	Fatty acids composition (% of total fatty acids)
		C14∶0	C16∶0	C16∶1	C18∶0	C18∶1	C18∶2	C18∶3
A	25.1	12.5	13.8	12.1	11.8	17.5	20.6	13.4
B	21.8	10.8	11.8	11.5	10.7	17.1	19.5	10.8
C	23.6	11.5	12.5	10.9	11.2	16.4	18.5	12.3

A: F/2 medium; B: F/2 medium without CO₂; C: 5% oyster shells pretreated with high-pressure homogenization and acetic acid (pH: 6.7).