Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase

Figure 4

Effects of pH on the stability (a) and activity (b) of A. japonicus LAB01 lipase. (a) Partially purified lipase samples were incubated for 90 min at 30°C in the presence of different buffers (100 mM): citrate phosphate (pH 3.0–6.0), sodium phosphate (pH 7.0), Tris-HCl (pH 8.0-9.0), and glycine-NaOH (pH 10.0), and the residual activity was measured using pNPP at pH 8.0 and 37°C. (b) Partially purified lipase samples were assayed in buffers B from pH 7.0 to 9.5 using pNPP as substrate at 37°C.

(a)

(b)