Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase

Table 3

Effect of ions on the activity/stability of the lipase from A.  japonicus LAB01.

Ions	Residual activity (%)
Mg^2+	112.97 ± 3.73
Li^+	109.22 ± 0.91
	104.03 ± 2.98
Na^+	107.61 ± 2.27
Ni^2+	70.42 ± 1.72
Cu^2+	59.84 ± 1.27
K^+	103.22 ± 2.36
Ca^2+	98.03 ± 1.58
	42.11 ± 5.08

Lipase samples were incubated in each ionic solution prepared in 200 mM Tris-HCl (pH 8.0) buffer at 30°C for 90 min. The remaining lipase activity relative to the non-ion containing control was determined on pNPP substrate at pH 8.0 and 37°C. Values are the average of assays carried out in triplicate.