Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines In Vitro
Table 2
Representative RayBio apoptosis assay overview (in part).
Protein name
U251 alone
ASC CM
UC-MSC-CM
Fold change 1
Fold change 2
Bad
10.05
181.61
203.00
18.07
20.20
Bax
192.00
265.05
363.00
1.38
1.89
Bcl-2
430.00
206.15
175.48
0.48
0.41
Bcl-w
350.00
310.80
286.03
0.89
0.82
BID
185.00
208.00
281.10
1.12
1.52
BIM
231.00
240.51
387.15
1.04
1.68
Caspase 3
235.00
932.60
881.00
3.97
3.75
Caspase 8
200.00
392.67
677.00
1.96
3.39
Fas
1271.50
1638.43
2100.05
1.29
1.65
FasL
274.50
269.46
177.42
0.98
0.65
p21
179.00
208.61
183.37
1.17
1.02
p27
240.00
333.80
238.20
1.39
0.99
p53
255.00
319.05
252.66
1.25
0.99
SMAC
407.50
1548.60
1535.00
3.80
3.77
Survivin
205.00
114.50
124.00
0.56
0.60
sTNF-R1
95.00
272.91
413.53
2.87
4.35
sTNF-R2
132.00
138.07
204.46
1.05
1.55
TNF-alpha
287.00
294.50
280.37
1.03
0.98
TNF-beta
246.00
349.48
306.32
1.42
1.25
XIAP
200.74
101.02
125.95
0.50
0.63
These readouts are the readouts after subtracting the background. Signal strength fold change larger than 1.5 or smaller than 0.7 is considered statistically significant. UC-MSCs and ASCs conditioned media led to significant overexpression of proapoptotic proteins such as bad, bax, BIM, and BID and significant downregulation of antiapoptotic proteins such as bcl-2, survivin, and XIAP.
