Establishment and Evaluation of Stable Cell Lines Inhibiting Foot-and-Mouth Disease Virus by RNA Interference

Figure 5

One amplification plot of real-time Q-RT-PCR analysis of the inhibition effects for the RNA replication of FMDV. Cells transfected with different expressing vectors at the second, fifth, tenth, twentieth, and thirtieth passages were infected with FMDV O/CHA/99. The real-time Q-RT-PCR analysis was performed on Stratagene real-time PCR Mx3000p system using the SYBR Premix Ex Taq Kit. Cells transfected with p3D1shRNA and p3D4shRNA showed an obviously lower level in RNA replication than the other groups.