Research Article
Establishment and Evaluation of Stable Cell Lines Inhibiting Foot-and-Mouth Disease Virus by RNA Interference
Table 1
Target sequences of shRNA in 3D gene of FMDV used in this study.
| | Name | Target sequence (O/CHA/99, AF506822) | Oligonucleotide sequence |
| | p3D1shRNA | 5′-AAGACTCGCATTGTCGACGTC-3′
(nt 7196–7216 in the 3D region) | 5′-GATCCGACTCGCATTGTCGACGTCTTCAAGAGAGACGTCGACAATGCGAGTCTTA-3′ (top strand)
5′-AGCTTAAGACTCGCATTGTCGACGTCTCTCTTGAAGACGTCGACAATGCGAGTCG-3′ (bottom strand) | | p3D2shRNA | 5′-AACAACATCTACGTGCTCTAC-3′
(nt 7598–7618 in the 3D region) | 5′-GATCCCAACATCTACGTGCTCTACTTCAAGAGAGTAGAGCACGTAGATGTTGTTA-3′ (top strand)
5′-AGCTTAACAACATCTACGTGCTCTACTCTCTTGAAGTAGAGCACGTAGATGTTGG-3′′ (bottom strand) | | p3D3shRNA | 5′-AACATCTACGTGCTCTACGCT-3′
(nt 7601–7621 in the 3D region) | 5′-GATCCCATCTACGTGCTCTACGCTTTCAAGAGA AGCGTAGAGCACGTAGATGTTA-3′ (top strand)
5′-AGCTTAACATCTACGTGCTCTACGCTTCTCTTGAAAGCGTAGAGCACGTAGATGG-3′′ (bottom strand) | | p3D4shRNA | 5′-AGACACTTCCACATGGACTA-3′
(nt 7828–7848 in the 3D region) | 5′-GATCCGACACTTCCACATGGACTATTCAAGAGATAGTCCATGTGGAAGTGTCTTA-3′ (top strand)
5′-AGCTTAAGACACTTCCACATGGACTATCTCTTGAATAGTCCATGTGGAAGTGTCG-3′′ (bottom strand) | | p3D5shRNA | 5′-AAGTTACAGATCACTTTACCT-3′
(nt 8026–8046 in the 3D region) | 5′-GATCCGTTACAGATCACTTTACCTTTCAAGAGA AGGTAAAGTGATCTGTAACTTA-3′ (top strand)
5′-AGCTTAAGTTACAGATCACTTTACCTTCTCTTGAAAGGTAAAGTGATCTGTAACG-3′′ (bottom strand) | | pshRNA | 5′-ACGGTGCCTTGCTCGCTAGAT-3′
(not homologous) | 5′-GATCCGGTGCCTTGCTCGCTAGATTTCAAGAGAATCTAGCGAGCAAGGCACCGT A-3′ (top strand)
5′-AGCTTACGGTGCCTTGCTCGCTAGATTCTCTTGAAATCTAGCGAGCAAGGCACCG-3′ (bottom strand) |
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Sense and antisense sequence are underlined.
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