Cardioprotective Effects of Osteopontin-1 during Development of Murine Ischemic Cardiomyopathy
Figure 4
Cellular and molecular changes in response to I/R. (a) Representative section of left ventricle after 7 days I/R in a WT-heart shows low interstitial macrophage accumulation (F4/80). (b) High accumulation of macrophages in areas of small nontransmural infarctions in OPN−/−-hearts (arrows). (c) Cell density of F4/80 positive macrophages in WT- compared to OPN−/−-hearts during I/R. (d) Differential macrophage cell count in WT-hearts revealed only few cells within areas of small infarctions (infarcted) in comparison to interstitial space. (e) In contrast, significantly more cells invaded areas of small infarctions in OPN−/−-mice than interstitial space. mRNA-induction of the galectin-3 (f), chemokines (g) CCL2 and (h) CCL3, and cytokines (i) TNF-α and (j) IL-10 in OPN−/−-hearts compared to WT-hearts during repetitive I/R. /group. Scale bars represent 50 m. RT-qPCR using Taqman and mRNA-expression is related to controls and GAPDH using comparative Ct-method. Bracket indicates between genotypes; indicates versus respective shams.
