Comparative Evaluation of Recombinant Protein Production in Different Biofactories: The Green Perspective

Table 1

Highest yields of the expression of the four selected recombinant pharmaceutical proteins in “traditional” heterologous production platforms.

Recombinant protein	Heterologous expression system	Highest expression level	Reference
hGAD65	Escherichia coli	12.5 mg/mL	[15]
	Saccharomyces cerevisiae	0.46 mg/mL	[27]
	Pichia pastoris	0.42 mg/mL	[27]
	Spodoptera frugiperda cells	0.02 mg/mL	[18]
	Mouse myeloma cells	1.67 mg/L	[16]
NVCP	Escherichia coli *	56 mg/L	[49]
	Pichia pastoris *	10 mg/L	[50]
	Spodoptera frugiperda cells*	125 mg/L	[29]
	Baby hamster kidney cells*	10^10 particles/mL	[51]
2G12	Hybridoma clones	10 pg/cell/day	[60]
hIL-6	Escherichia coli	7.5 mg/mL	[102]
	Pichia pastoris	0.28 mg/mL	[104]
	Spodoptera frugiperda cells	0.001 mg/mL	[106]

∗Reported values are the highest yield data of purified or partially purified recombinant protein because of the absence of expression data.