Negative Regulation of GADD34 on Myofibroblasts during Cutaneous Wound Healing

Figure 2

Myofibroblast differentiation was downregulated by GADD34.   α-SMA expression in granulation tissues from WT and GADD34^−/− mice at various time points after injury was analyzed by immunofluorescence assay using antibodies against α-SMA (red) and DAPI (blue). (a) Low magnification of wound site of each indicated time point was shown. Selected area by white dotted line means granulation tissue. Scale bar: 100 μm and (b) the percentages of α-SMA⁺ area within granulation tissue at each time point were quantified from 4 sections by using image J software. (c) High magnification of wound site was shown. Scale bar: 50 μm and (d) the number of α-SMA⁺ cells was counted per 40000 μm² from 4 sections. Bar graph of relative intensity shown is mean ± SD. , .

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