Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture
Figure 6
Analysis of all long-term cultured spermatogonia by real-time PCR. PCA shows that htFibs, hESCs, short-term cultured hSSCs, and a group of long-term cultured hSCCs (group I) clearly separate. (b) Bar plots of long-term cultured spermatogonia (group I) in comparison to short-term cultured spermatogonia, hESCs, and htFibs: (a) For germ cell-associated genes, (b) pluripotency-associated genes, and (c) germ cell-associated genes selected from microarray study. Coloring: htFibs: red; hESCs: grey; short-term cultured spermatogonia from patient 219: black; long-term cultured spermatogonia from group I: green; long-term cultured spermatogonia from group II: blue.
