Superoxide Dismutase 1 Loss Disturbs Intracellular Redox Signaling, Resulting in Global Age-Related Pathological Changes
Figure 2
The skin and cellular phenotypes of Sod1 −/− mice. (a) The hematoxylin and eosin staining of the back skin of Sod1 −/− and Sod1 +/+ mice. Sod1 −/− mice (5 months of age) were transdermally treated with 1% L-ascorbyl 2-phosphate 6-palmitate trisodium salt (APPS) for 4 weeks. (b) Dermal fibroblasts were dissected from Sod1 −/− neonates at 5 days of age. The cells were cultured with or without 10 μM APPS for 48 h under 20% O2. (c) The intracellular ROS levels in Sod1 −/− fibroblasts treated with 10 μM APPS were measured by examining the presence of CM-H2DCFDA. The scale bars represent 100 μm.
