Table 4: (a) Genome GO:0051082 (unfolded protein binding). (b) Real-time PCR array of chaperone and heat shock genes.
(a)

Entrez IDSymbolGene nameAverage
intensity
Stress versus no stressMDMA versus no MDMA
No MDMAMDMANo stressStress
−Fold
change
value−Fold
change
value −Fold
change
value−Fold
change
value

361384Dnajb1DnaJ (Hsp40) homolog, subfamily B, member 1363.161.020.81−1.090.502.180.001.960.00
65028Dnaja1DnaJ (Hsp40) homolog, subfamily A, member 11222.761.040.431.030.511.450.001.440.00
295549Dnajb4DnaJ (Hsp40) homolog, subfamily B, member 4668.671.020.731.010.871.420.001.400.00
300721Dnaja4DnaJ (Hsp40) homolog, subfamily A, member 4232.201.070.48−1.100.211.390.011.180.01
288620Cct6achaperonin subunit 6a (zeta)395.911.030.73−1.020.501.370.001.300.00
360734Dnajb11DnaJ (Hsp40) homolog, subfamily B, member 11472.491.010.93−1.020.751.360.001.330.00
29345Serpinh1Serine (or cysteine) peptidase inhibitor, clade H, member 1469.00−1.040.77−1.120.471.360.151.260.00
295230Cct3chaperonin subunit 3 (gamma)686.331.030.62−1.060.261.350.001.240.00
311456MkksMcKusick-Kaufman syndrome protein320.401.060.331.020.811.350.001.300.00
64202CalrCalreticulin1882.721.030.63−1.050.291.320.001.220.00
63868Hspd1Heat shock protein 1 (chaperonin)1509.641.010.83−1.000.981.280.011.260.00
29374Cct4Chaperonin subunit 4 (delta)662.361.030.63−1.040.451.280.001.200.00
25719Scg5Secretogranin V2031.481.050.34−1.000.941.260.001.190.00
362862Tra1Tumor rejection antigen gp962797.041.030.631.040.331.250.011.260.00
24818Tcp1t-complex protein 1474.451.020.80−1.010.811.240.001.200.01
301252Hsp90ab1Heat shock protein 90 kDa alpha (cytosolic), class B member 14755.121.010.72−1.030.351.240.001.180.00
24468Hspa8Heat shock protein 83661.991.020.841.060.331.220.031.270.00
294864Cct5Chaperonin subunit 5 (epsilon)1223.731.040.451.010.821.220.001.180.00
368044Atp6v1g2ATPase, H+ transporting, V1 subunit G isoform 21494.801.020.72−1.030.381.220.001.150.00
294236Gtf2h4General transcription factor II H, polypeptide 4106.291.080.39−1.040.361.210.011.080.22
84026Dnaja2DnaJ (Hsp40) homolog, subfamily A, member 21040.621.050.471.060.061.210.001.230.00
619393Dnajc12DnaJ (Hsp40) homolog, subfamily C, member 12367.661.060.29−1.010.721.210.001.120.03
89811VegfbVascular endothelial growth factor B330.14−1.030.66−1.040.331.220.011.230.00
299313UxtUbiquitously expressed transcript616.75−1.050.511.040.201.290.00−1.190.00
29427Aif1Allograft inflammatory factor 1172.49−1.070.471.250.001.390.001.620.00

Individual members of the gene ontology category Unfolded Binding Protein are shown. The average intensity of all genes shown is above threshold. Fold changes and values beyond the cutoff for significance are in bold. For clarity, genes that do not reach significance in any category are not shown. Genes induced by MDMA are separated by a free line.
(b)

Gene symbolFold regulation

Hspa52.6069
Cct52.5859
Dnajb12.3823
Cryab2.3305
Hspb12.2668
Hspa22.215
Bag32.0596
Dnaja41.8908
Cct71.8412
Dnajb111.8159
Hspa141.8117
Dnajb41.7724
Ldha1.7399
Dnaja11.6982
Hsph11.6806
Dnajc71.6537
Hsp90ab11.5955
Cct6a1.5808
Hsp90aa11.5609
Cct21.5112
Hsp90b11.5025
Serpinh11.4973
Dnaja21.4346
Hspa4l1.4198

Real-time PCR validation of chaperone and heat shock gene upregulation by MDMA exposure (2 rats) versus unstressed untreated rats (3 rats). Fold change (2−ΔΔCt) is the normalized gene expression (2−ΔCt) in the test sample divided by the normalized gene expression (2−ΔCt) in the control sample. Fold regulation represents fold change results in a biologically meaningful way. Fold change values greater than one indicate a negative or downregulation and the fold regulation is the negative inverse of the fold change. Shown are positive changes in fold regulation by MDMA of at least 1.4 over controls.